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loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-5629 size-full\" src=\"https:\/\/dev.academist-cf.com\/journal\/wp-content\/uploads\/2017\/08\/001n.jpg\" alt=\"\" width=\"600\" height=\"450\" srcset=\"https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/08\/001n.jpg 600w, https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/08\/001n-300x225.jpg 300w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><\/p>\n<h3>\u30d3\u30d5\u30a3\u30ba\u30b9\u83cc\u306e\u7cd6\u4ee3\u8b1d\u9175\u7d20\u306e\u5f62\u304b\u3089\u308f\u304b\u308b\u3053\u3068<\/h3>\n<p>\u79c1\u305f\u3061\u306e\u7814\u7a76\u5ba4\u3067\u306f\u3001\u30d3\u30d5\u30a3\u30ba\u30b9\u83cc\u304c\u30d2\u30c8\u30df\u30eb\u30af\u30aa\u30ea\u30b4\u7cd6\u3084\u30e0\u30c1\u30f3\uff08\u7cd6\u30bf\u30f3\u30d1\u30af\u8cea\uff09\u3092\u5206\u89e3\u3057\u3066\u4ee3\u8b1d\u3059\u308b\u4e00\u9023\u306e\u9175\u7d20\u3084\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u69cb\u9020\u89e3\u6790\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7acb\u4f53\u69cb\u9020\u304b\u3089\u660e\u3089\u304b\u306b\u306a\u308b\u3053\u3068\u306f\u591a\u304f\u3001\u305f\u3068\u3048\u3070\u3001\u9175\u7d20\u306e\u53cd\u5fdc\u6a5f\u69cb\u3084\u57fa\u8cea\u306e\u8a8d\u8b58\u306e\u69d8\u5b50\u304c\u8a73\u7d30\u306b\u308f\u304b\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n<p>\u30d3\u30d5\u30a3\u30ba\u30b9\u83cc\u306b\u7279\u5fb4\u7684\u306b\u898b\u3089\u308c\u308b\u9175\u7d20\u3067\u3042\u308b\u30db\u30b9\u30db\u30b1\u30c8\u30e9\u30fc\u30bc\u306f\u3001\u3053\u306e\u83cc\u304c\u4ed6\u3068\u9055\u3063\u3066\u52b9\u7387\u306e\u826f\u3044\u7cd6\u4ee3\u8b1d\u7d4c\u8def\u3092\u6709\u3059\u308b\u305f\u3081\u306e\u9375\u3068\u306a\u308b\u9175\u7d20\u306a\u306e\u3067\u3059\u304c\u3001\u88dc\u9175\u7d20\u3068\u3057\u3066\u30c1\u30a2\u30df\u30f3\uff08\u30d3\u30bf\u30df\u30f3B<sub>1<\/sub>\uff09\u3092\u5229\u7528\u3057\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u7acb\u4f53\u69cb\u9020\u304b\u3089\u3001\u30c1\u30a2\u30df\u30f3\u306b\u7d50\u5408\u3057\u305f\u53cd\u5fdc\u4e2d\u9593\u4f53\uff08\u30a2\u30bb\u30c1\u30ebTPP\u4e2d\u9593\u4f53\uff09\u306b\u30ea\u30f3\u9178\u304c\u76f4\u63a5\u53cd\u5fdc\u3057\u3066\u9ad8\u30a8\u30cd\u30eb\u30ae\u30fc\u5316\u5408\u7269\uff08\u30a2\u30bb\u30c1\u30eb\u30ea\u30f3\u9178\uff09\u3092\u751f\u7523\u3059\u308b\u30e1\u30ab\u30cb\u30ba\u30e0\u304c\u660e\u3089\u304b\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u307e\u305f\u3001NagBb\u3067\u306f\u3001N-\u30a2\u30bb\u30c1\u30eb\u30ac\u30e9\u30af\u30c8\u30b5\u30df\u30f3\u306e\u8a8d\u8b58\u306b\u3001\u91d1\u5c5e\u30a4\u30aa\u30f3\u304c\u95a2\u308f\u3063\u3066\u3044\u308b\u3053\u3068\u3082\u660e\u3089\u304b\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u7cd6\u306eN-\u30a2\u30bb\u30c1\u30eb\u57fa\u306e\u8a8d\u8b58\u306b\u91d1\u5c5e\u30a4\u30aa\u30f3\u304c\u95a2\u308f\u308b\u3068\u3044\u3046\u3053\u3068\u306f\u3053\u308c\u307e\u3067\u77e5\u3089\u308c\u3066\u304a\u3089\u305a\u3001\u30d3\u30d5\u30a3\u30ba\u30b9\u83cc\u306e\u9175\u7d20\u3092\u8abf\u3079\u308b\u3068\u3001\u65b0\u3057\u3044\u3053\u3068\u304c\u3044\u308d\u3044\u308d\u3068\u308f\u304b\u3063\u3066\u304d\u307e\u3059\u3002<\/p>\n<figure id=\"attachment_5611\" aria-describedby=\"caption-attachment-5611\" style=\"width: 600px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-5611\" src=\"https:\/\/dev.academist-cf.com\/journal\/wp-content\/uploads\/2017\/08\/002-7.jpg\" alt=\"\" width=\"600\" height=\"450\" srcset=\"https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/08\/002-7.jpg 600w, https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/08\/002-7-300x225.jpg 300w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption id=\"caption-attachment-5611\" class=\"wp-caption-text\">\u30db\u30b9\u30db\u30b1\u30c8\u30e9\u30fc\u30bc\u3068NagBb\u306e\u69cb\u9020<\/figcaption><\/figure>\n<p>\u30d3\u30d5\u30a3\u30ba\u30b9\u83cc\u306b\u306f\u4ed6\u306e\u5fae\u751f\u7269\u306f\u6301\u3063\u3066\u3044\u306a\u3044\u3088\u3046\u306a\u30e6\u30cb\u30fc\u30af\u306a\u9175\u7d20\u304c\u591a\u304f\u5b58\u5728\u3057\u3066\u304a\u308a\u3001\u79c1\u306f\u3001\u3053\u308c\u3089\u306e\u9175\u7d20\u304c\u3069\u306e\u3088\u3046\u306a\u5206\u5b50\u9032\u5316\u3092\u9042\u3052\u3066\u73fe\u5728\u306e\u3088\u3046\u306a\u5f62\u3084\u6a5f\u80fd\u3092\u6301\u3064\u306b\u81f3\u3063\u305f\u304b\u306b\u3064\u3044\u3066\u8208\u5473\u3092\u6301\u3063\u3066\u3044\u307e\u3059\u3002\u305f\u3068\u3048\u3070\u3001\u30db\u30b9\u30db\u30b1\u30c8\u30e9\u30fc\u30bc\u306f\u30da\u30f3\u30c8\u30fc\u30b9\u30ea\u30f3\u9178\u7d4c\u8def\u3084\u30ab\u30eb\u30d3\u30f3\u56de\u8def\u306a\u3069\u3067\u50cd\u3044\u3066\u3044\u308b\u30c8\u30e9\u30f3\u30b9\u30b1\u30c8\u30e9\u30fc\u30bc\u3068\u3044\u3046\u9175\u7d20\u306e\u6d3b\u6027\u90e8\u4f4d\u304c\u5909\u5316\u3057\u3066\u51fa\u6765\u3066\u3044\u308b\u3053\u3068\u3001NagBb\u306f\u540c\u69d8\u306b\u30e0\u30c1\u30f3\u306e\u7cd6\u4ee3\u8b1d\u306b\u95a2\u308f\u3063\u3066\u3044\u308bEngBF\u3068\u5c11\u3057\u69cb\u9020\u304c\u4f3c\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002\u307e\u305f\u3001GLNBP\u306f\u03b2-\u30ac\u30e9\u30af\u30c8\u30b7\u30c0\u30fc\u30bc\u3068\u3044\u3046\u9175\u7d20\u3068\u3001NahK\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u30ea\u30f3\u9178\u57fa\u3092\u4ed8\u52a0\u3059\u308b\u9175\u7d20\u3067\u3042\u308b\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bc\u3068\u3001\u305d\u308c\u305e\u308c\u9032\u5316\u7684\u8d77\u6e90\u3092\u5171\u901a\u306b\u6301\u3063\u3066\u3044\u308b\u3068\u63a8\u6e2c\u3055\u308c\u307e\u3059\u3002\u30d3\u30d5\u30a3\u30ba\u30b9\u83cc\u306f\u3001\u8178\u5185\u3067\u751f\u304d\u629c\u304f\u306e\u306b\u5fc5\u8981\u306a\u3001\u7279\u6b8a\u306a\u4ee3\u8b1d\u7cfb\u3092\u5f97\u308b\u305f\u3081\u306b\u3001\u3055\u307e\u3056\u307e\u306a\u8d77\u6e90\u3092\u6301\u3064\u9175\u7d20\u3092\u7d44\u307f\u5408\u308f\u305b\u3066\u5229\u7528\u3057\u3066\u3044\u308b\u306e\u3067\u306f\u306a\u3044\u304b\u3068\u8003\u3048\u3066\u3044\u307e\u3059\u3002<\/p>\n<h3>\u307e\u3063\u305f\u304f\u7570\u306a\u308b2\u7a2e\u985e\u306eLNB\u30a2\u30fc\u30bc<\/h3>\n<p>\u30d3\u30d5\u30a3\u30ba\u30b9\u83cc\u306e\u3046\u3061<i>Bifidobacterium bifidum<\/i>\u3068\u547c\u3070\u308c\u308b\u7a2e\u306f\u3001GH20\u306b\u5c5e\u3059\u308bLNB\u30a2\u30fc\u30bc\uff08LnbB\uff09\u3092\u6301\u3063\u3066\u3044\u307e\u3059\u304c\u3001<i>Bifidobacterium longum<\/i>\u3067\u306fGH136\u3068\u3044\u3046\u65b0\u3057\u3044\u30d5\u30a1\u30df\u30ea\u30fc\u306b\u5c5e\u3059\u308bLnbX\u3068\u3044\u3046LNB\u30a2\u30fc\u30bc\u3092\u6301\u3063\u3066\u3044\u307e\u3059\u3002\u3053\u306e2\u3064\u306e\u9175\u7d20\u306f\u3069\u3061\u3089\u3082\u30d2\u30c8\u30df\u30eb\u30af\u30aa\u30ea\u30b4\u7cd6\u304b\u3089LNB\u3092\u5207\u308a\u51fa\u3059\u3068\u3044\u3046\u540c\u3058\u6a5f\u80fd\u3092\u6301\u3063\u3066\u3044\u308b\u306e\u3067\u3059\u304c\u3001\u305d\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u3057\u3066\u306e\u69cb\u9020\u3084\u53cd\u5fdc\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u306f\u307e\u3063\u305f\u304f\u7570\u306a\u308a\u307e\u3059\u3002<\/p>\n<figure id=\"attachment_5612\" aria-describedby=\"caption-attachment-5612\" style=\"width: 600px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-5612\" src=\"https:\/\/dev.academist-cf.com\/journal\/wp-content\/uploads\/2017\/08\/003-5.jpg\" alt=\"\" width=\"600\" height=\"450\" srcset=\"https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/08\/003-5.jpg 600w, https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/08\/003-5-300x225.jpg 300w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption id=\"caption-attachment-5612\" class=\"wp-caption-text\">2\u7a2e\u985e\u306eLNB\u30a2\u30fc\u30bc\u306e\u69cb\u9020<\/figcaption><\/figure>\n<p>LnbB\u306f\u3001\u7cd6\u8cea\u5206\u89e3\u9175\u7d20\u3067\u3088\u304f\u898b\u3089\u308c\u308b(\u03b2\/\u03b1)<sub>8<\/sub>\u30d0\u30ec\u30eb\u3068\u3044\u3046\u30d5\u30a9\u30fc\u30eb\u30c9\uff08\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6298\u308a\u305f\u305f\u307f\u30d1\u30bf\u30fc\u30f3\uff09\u3092\u6301\u3061\u3001\u540c\u3058GH20\u306b\u5c5e\u3059\u308b\u03b2-N-\u30a2\u30bb\u30c1\u30eb\u30d8\u30ad\u30bd\u30b5\u30df\u30cb\u30c0\u30fc\u30bc\u3068\u3044\u3046\u9175\u7d20\u306e\u30dd\u30b1\u30c3\u30c8\u304c\u5e83\u304c\u308b\u3053\u3068\u306b\u3088\u308aLNB\u304c\u7d50\u5408\u3067\u304d\u308b\u3088\u3046\u306b\u5206\u5b50\u9032\u5316\u3057\u3066\u304d\u305f\u3053\u3068\u304c\u63a8\u5bdf\u3055\u308c\u307e\u3059\u3002\u307e\u305f\u3001LNB\u306e\u5207\u65ad\u306b\u306f\u3001\u57fa\u8cea\u306e\u306a\u304b\u306eN-\u30a2\u30bb\u30c1\u30eb\u57fa\u81ea\u8eab\u304c\u53cd\u5fdc\u3059\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u4e00\u65b9\u3001LnbX\u306f\u3001\u03b2-\u30d8\u30ea\u30c3\u30af\u30b9\u3068\u547c\u3070\u308c\u308b\u30d5\u30a9\u30fc\u30eb\u30c9\u3092\u6301\u3061\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u306a\u304b\u306e418\u756a\u76ee\u306e\u30a2\u30b9\u30d1\u30e9\u30ae\u30f3\u9178\u6b8b\u57fa\u304c\u53cd\u5fdc\u3057\u3066\u3001\u6c34\u5206\u5b50\u304c\u305d\u306e\u53cd\u5fdc\u306b\u95a2\u308f\u308b\u3053\u3068\u3082\u308f\u304b\u308a\u307e\u3057\u305f\u3002LnbX\u306e\u9032\u5316\u7684\u8d77\u6e90\u306b\u3064\u3044\u3066\u306f\u3088\u304f\u308f\u304b\u3089\u306a\u3044\u306e\u3067\u3059\u304c\u3001\u9762\u767d\u3044\u3053\u3068\u306b\u3001\u30d5\u30a1\u30fc\u30b8\u7531\u6765\u306e\u30c6\u30a4\u30eb\u30b9\u30d1\u30a4\u30af\u30bf\u30f3\u30d1\u30af\u8cea\u306a\u3069\u3068\u3082\u5f62\u304c\u5c11\u3057\u4f3c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u3053\u3068\u306f\u3001<i>Bifidobacterium<\/i>\u5c5e\u304c\u4e73\u5150\u306e\u8178\u5185\u74b0\u5883\u3067\u30d2\u30c8\u30df\u30eb\u30af\u30aa\u30ea\u30b4\u7cd6\u5206\u89e3\u3092\u5206\u89e3\u3059\u308b\u306b\u3042\u305f\u308a\u3001\u307e\u3063\u305f\u304f\u7570\u306a\u308b\u30bf\u30a4\u30d7\u306eLNB\u30a2\u30fc\u30bc\u3092\u5225\u3005\u306b\u7372\u5f97\u3057\u305f\u3053\u3068\u3092\u793a\u3057\u3066\u304a\u308a\u3001\u975e\u5e38\u306b\u8208\u5473\u6df1\u3044\u4e8b\u5b9f\u3060\u3068\u8003\u3048\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u30d3\u30d5\u30a3\u30ba\u30b9\u83cc\u306b\u9650\u3089\u305a\u3001\u8178\u5185\u7d30\u83cc\u306f\u3001\u98df\u7269\u3084\u5bbf\u4e3b\u7531\u6765\u306e\u7cd6\u8cea\u3092\u596a\u3044\u5408\u3046\u74b0\u5883\u3067\u7af6\u4e89\u3057\u3064\u3064\u3001\u5bbf\u4e3b\u3068\u306e\u5171\u751f\u3092\u306f\u304b\u3063\u3066\u3044\u308b\u3068\u3082\u8a00\u3048\u307e\u3059\u3002\u305d\u3053\u3067\u306f\u591a\u69d8\u306a\u7cd6\u8cea\u5206\u89e3\u30c4\u30fc\u30eb\u3092\u6301\u3064\u5fae\u751f\u7269\u304c\u6804\u990a\u72b6\u614b\u306e\u5909\u5316\u306b\u5fdc\u3058\u3066\u5897\u6b96\u306e\u30c1\u30e3\u30f3\u30b9\u3092\u5e83\u3052\u3089\u308c\u308b\u306e\u3067\u3001\u65b0\u3057\u3044\u9175\u7d20\u304c\u3069\u3093\u3069\u3093\u73fe\u308c\u308b\u3001\u9175\u7d20\u306e\u5206\u5b50\u9032\u5316\u306e\u5b9f\u9a13\u5834\u306e\u3088\u3046\u306a\u5834\u6240\u306a\u306e\u3067\u306f\u306a\u3044\u304b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/p>\n<p><strong>\u53c2\u8003\u6587\u732e<\/strong><br \/>\nSuzuki R et al &amp; Fushinobu S (2010). Crystal structures of phosphoketolase: thiamine diphosphate-dependent dehydration mechanism. J. Biol. Chem. 285(44), 34279-34287, doi: 10.1074\/jbc.M110.156281<br \/>\nSato M et al &amp; Fushinobu S (2017). The first crystal structure of a family 129 glycoside hydrolase from a probiotic bacterium reveals critical residues and metal cofactors. J. Biol. Chem. 292(29), 12126-12138. doi: 10.1074\/jbc.M117.777391<br \/>\nYamada C et al &amp; Fushinobu S (2017). Molecular insight into evolution of symbiosis between breast-fed infants and a member of the human gut microbiome Bifidobacterium longum. Cell Chem. Biol. 24(4), 515-524 doi: 10.1016\/j.chembiol.2017.03.012<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u8178\u5185\u7d30\u83cc\u306f\u65b0\u3057\u3044\u7cd6\u4ee3\u8b1d\u9175\u7d20\u306e\u5b9d\u5eab \u5065\u5eb7\u306a\u30d2\u30c8\u306e\u8178\u5185\u306b\u306f\u6570\u767e\u7a2e\u4ee5\u4e0a\u306e\u8178\u5185\u7d30\u83cc\u304c\u68f2\u3093\u3067\u3044\u308b\u3068\u8a00\u308f\u308c\u307e\u3059\u3002\u8178\u5185\u74b0\u5883\u306f\u5acc\u6c17\u7684\u306a\u306e\u3067\u3001\u9178\u7d20\u3092\u4f7f\u308f\u306a\u3044\u7cd6\u8cea\u306e\u767a\u9175\u306b\u305d\u306e\u4e3b\u306a\u30a8\u30cd\u30eb\u30ae\u30fc\u6e90\u3092\u983c\u3063\u3066\u3044\u307e\u3059\u3002\u3068\u3053\u308d\u304c\u3001\u30d2\u30c8 [&hellip;]<\/p>\n","protected":false},"author":187,"featured_media":5612,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[3],"tags":[50,15,109],"class_list":["post-5614","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-outcome","tag-50","tag-biology","tag-109","has_thumb"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/posts\/5614","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/users\/187"}],"replies":[{"embeddable":true,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=5614"}],"version-history":[{"count":0,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/posts\/5614\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/media\/5612"}],"wp:attachment":[{"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=5614"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=5614"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=5614"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}