{"id":4197,"date":"2017-04-17T18:00:47","date_gmt":"2017-04-17T09:00:47","guid":{"rendered":"https:\/\/dev.academist-cf.com\/journal\/?p=4197"},"modified":"2018-12-25T17:34:27","modified_gmt":"2018-12-25T08:34:27","slug":"%e8%9a%95%e6%a5%ad%e9%9d%a9%e5%91%bd%e3%81%ae%e5%ae%9f%e7%8f%be%e3%82%92%e7%9b%ae%e6%8c%87%e3%81%97%e3%81%a6-%e5%be%b3%e5%b3%b6%e5%a4%a7%e3%83%bb%e4%bc%8a%e8%97%a4%e6%95%99%e6%8e%88%e3%81%ae","status":"publish","type":"post","link":"https:\/\/academist-cf.com\/journal\/?p=4197","title":{"rendered":"\u8695\u696d\u9769\u547d\u306e\u5b9f\u73fe\u3092\u76ee\u6307\u3057\u3066 &#8211; \u5fb3\u5cf6\u5927\u30fb\u4f0a\u85e4\u6559\u6388\u306e\u30b5\u30a4\u30a8\u30f3\u30b9\u30ab\u30d5\u30a7\u958b\u50ac\u30ec\u30dd\u30fc\u30c8"},"content":{"rendered":"<p>2016\u5e7410\u6708\u306b\u300c<a href=\"https:\/\/academist-cf.com\/projects\/?id=31\">\u7d44\u63db\u3048\u30ab\u30a4\u30b3\u3067\u300c\u30ea\u30bd\u30bd\u30fc\u30e0\u75c5\u300d\u306e\u6cbb\u7642\u85ac\u3092\u4f5c\u308a\u305f\u3044\uff01<\/a>\u300d\u3067\u76ee\u6a19\u91d1\u984d\u3092\u9054\u6210\u3057\u305f\u3001\u5fb3\u5cf6\u5927\u5b66\u30fb\u4f0a\u85e4\u5b5d\u53f8\u6559\u6388\u306e\u4e3b\u50ac\u3059\u308b\u30b5\u30a4\u30a8\u30f3\u30b9\u30ab\u30d5\u30a7\u304c2017\u5e744\u67081\u65e5\uff08\u571f\uff09\u306b\u6771\u4eac\u3067\u958b\u50ac\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n<p>\u4eca\u56de\u306e\u30b5\u30a4\u30a8\u30f3\u30b9\u30ab\u30d5\u30a7\u306b\u306f\u3001\u4f0a\u85e4\u5148\u751f\u3068\u5171\u540c\u7814\u7a76\u8005\u306e\u702c\u7b52\u79c0\u6a39\u5148\u751f\uff08\u8fb2\u7814\u6a5f\u69cb \u30ab\u30a4\u30b3\u6a5f\u80fd\u6539\u5909\u6280\u8853\u958b\u767a\u30e6\u30cb\u30c3\u30c8 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src=\"https:\/\/dev.academist-cf.com\/journal\/wp-content\/uploads\/2017\/04\/Screen-Shot-2017-04-09-at-14.11.33.png\" alt=\"\" width=\"600\" height=\"373\" srcset=\"https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/04\/Screen-Shot-2017-04-09-at-14.11.33.png 465w, https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/04\/Screen-Shot-2017-04-09-at-14.11.33-300x186.png 300w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption id=\"caption-attachment-4198\" class=\"wp-caption-text\">\u702c\u7b52\u79c0\u6a39\u5148\u751f\uff08\u53f3\uff09<\/figcaption><\/figure>\n<p class=\"p1\"><span class=\"s1\">\u702c\u7b52\u5148\u751f\u306e\u8b1b\u6f14\u3067\u306f\u3001\u4f0a\u85e4\u5148\u751f\u306e\u7814\u7a76\u3092\u7406\u89e3\u3059\u308b\u306e\u306b\u3082\u5f79\u7acb\u3064\u3001<br \/>\n<\/span><span class=\"s1\">1. \u305d\u3082\u305d\u3082\u30ab\u30a4\u30b3\u3068\u306f\u4f55\u304b<br \/>\n<\/span><span class=\"s1\">2. \u4eca\u307e\u3067\u306e\u30ab\u30a4\u30b3\u306e\u5229\u7528\u6cd5<br \/>\n<\/span><span class=\"s1\">3. \u4eca\u5f8c\u306e\u9769\u65b0\u7684\u30ab\u30a4\u30b3\u5229\u7528\u6cd5<br \/>\n<\/span><span class=\"s1\">\u3068\u3044\u3046\u304a\u8a71\u304c\u30e1\u30a4\u30f3\u3067\u7d39\u4ecb\u3055\u308c\u3066\u3044\u307e\u3057\u305f\u3002<\/span><\/p>\n<p class=\"p1\"><span class=\"s1\">\u4eca\u307e\u3067\u7d79\u7cf8\u3092\u751f\u7523\u3059\u308b\u305f\u3081\u306b\u990a\u8695\u696d\u3067\u5229\u7528\u3057\u3066\u304d\u305f\u30ab\u30a4\u30b3\u3067\u3059\u304c\u3001\u8fd1\u5e74\u907a\u4f1d\u5b50\u6539\u5909\u3092\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001<\/span><span class=\"s1\">\u6709\u7528\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u3084\u65b0\u7e4a\u7dad\u3092\u751f\u7523\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u3053\u308c\u306f\u3001\u4eca\u307e\u3067\u4e3b\u306b\u8863\u6599\u5206\u91ce\u306b\u7528\u3044\u3089\u308c\u3066\u304d\u305f\u30ab\u30a4\u30b3\u3092\u300c\u533b\u7642\u300d\u3084\u300c\u5de5\u696d\u300d\u306b\u3082\u5229\u7528\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308b\u3053\u3068\u3092\u610f\u5473\u3057\u307e\u3059\u3002\u702c\u7b52\u5148\u751f\u306f\u3053\u308c\u3092\u300c\u8695\u696d\u9769\u547d\u300d\u3068\u547c\u3073\u3001\u65b0\u7523\u696d\uff08\u65b0\u8695\u696d\uff09\u3092\u5275\u308a\u51fa\u3059\u3079\u304f\u73fe\u5728\u7814\u7a76\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002<\/span><\/p>\n<p class=\"p1\"><span class=\"s1\">\u306a\u305c\u4ed6\u306e\u6606\u866b\u3067\u306f\u306a\u304f\u300c\u30ab\u30a4\u30b3\u300d\u304c\u3053\u306e\u3088\u3046\u306a\u53ef\u80fd\u6027\u3092\u79d8\u3081\u3066\u3044\u308b\u306e\u3067\u3057\u3087\u3046\u304b\u3002\u305d\u306e\u7406\u7531\u306f\u30ab\u30a4\u30b3\u304c\u7d21\u3050\u7d79\u7cf8\u306e\u6027\u8cea\u306b\u3042\u308a\u307e\u3059\u3002<\/span><\/p>\n<figure id=\"attachment_4199\" aria-describedby=\"caption-attachment-4199\" style=\"width: 600px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4199 size-full\" src=\"https:\/\/dev.academist-cf.com\/journal\/wp-content\/uploads\/2017\/04\/Screen-Shot-2017-04-09-at-14.15.17.png\" alt=\"\" width=\"600\" height=\"484\" srcset=\"https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/04\/Screen-Shot-2017-04-09-at-14.15.17.png 600w, https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/04\/Screen-Shot-2017-04-09-at-14.15.17-300x242.png 300w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption id=\"caption-attachment-4199\" class=\"wp-caption-text\">\u7d79\u7cf8\u306f\u300c\u30bb\u30ea\u30b7\u30f3\u300d\u3068\u300c\u30d5\u30a3\u30d6\u30ed\u30a4\u30f3\u300d\u3068\u3044\u3046\u6027\u8cea\u306e\u7570\u306a\u308b2\u3064\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u304b\u3089\u69cb\u6210\u3055\u308c\u308b<\/figcaption><\/figure>\n<p>\u6c34\u6eb6\u6027\u3067\u3042\u308b\u30bb\u30ea\u30b7\u30f3\u90e8\u306b\u6709\u7528\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u542b\u3081\u308b\u3088\u3046\u30ab\u30a4\u30b3\u306b\u907a\u4f1d\u5b50\u6539\u5909\u3092\u884c\u3048\u3070\u3001\u7e6d\u3092\u6c34\u306b\u6d78\u3059\u3060\u3051\u3067\u6bd4\u8f03\u7684\u593e\u96d1\u7269\u304c\u5c11\u306a\u3044\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u307e\u305f\u3001\u30bb\u30ea\u30b7\u30f3\u306b\u5305\u307e\u308c\u3066\u5b58\u5728\u3057\u3066\u3044\u308b\u30d5\u30a3\u30d6\u30ed\u30a4\u30f3\u90e8\u306b\u6539\u5909\u3092\u884c\u3046\u3068\u3001\u7e4a\u7dad\u3068\u3057\u3066\u306e\u7279\u6027\u3092\u5909\u5316\u3055\u305b\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u305f\u3068\u3048\u3070\u30012008\u5e74\u9803\u304b\u3089\u8a71\u984c\u306b\u306a\u3063\u3066\u3044\u308b\u300c\u5149\u308b\u7d79\u7cf8\u300d\u306f\u3001\u30d5\u30a3\u30d6\u30ed\u30a4\u30f3\u90e8\u306b\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8ceaGFP\u3092\u542b\u3080\u3088\u3046\u30ab\u30a4\u30b3\u306b\u907a\u4f1d\u5b50\u6539\u5909\u3092\u884c\u3063\u3066\u5f97\u3089\u308c\u305f\u3082\u306e\u3067\u3059\u3002\u30bb\u30ea\u30b7\u30f3\u3068\u30d5\u30a3\u30d6\u30ed\u30a4\u30f3\u3001\u3053\u306e\u305d\u308c\u305e\u308c\u306e\u6027\u8cea\u306b\u3088\u308a\u3001\u7e6d\uff08\u7d79\u7cf8\uff09\u3092\u5229\u7528\u3057\u305f\u533b\u7642\u3084\u5de5\u696d\u3078\u306e\u5fdc\u7528\u304c\u53ef\u80fd\u306b\u306a\u3063\u3066\u3044\u308b\u306e\u3067\u3059\u3002<\/p>\n<p>\u307e\u305f\u3001\u30ab\u30a4\u30b3\u4e00\u982d\u3092\u80b2\u3066\u30660.5 g\u306e\u7e6d\u3092\u5f97\u308b\u306e\u306b\u5fc5\u8981\u306a\u30a8\u30b5\uff08\u6851\u306e\u8449\uff09\u306f\u7d0420\uff47\u3068\u3055\u308c\u3066\u304a\u308a\u3001\u304a\u91d1\u3067\u3044\u3046\u3068\u7d042\u5186\u306b\u76f8\u5f53\u3057\u307e\u3059\u3002\u4ed6\u306e\u88fd\u85ac\u6280\u8853\u3067\u7528\u3044\u3089\u308c\u308b\u54fa\u4e73\u985e\u7d30\u80de\u306e\u57f9\u990a\u3088\u308a\u3082\u306f\u308b\u304b\u306b\u5b89\u3044\u30b3\u30b9\u30c8\u3067\u904b\u7528\u304c\u3067\u304d\u308b\u3068\u3044\u3046\u70b9\u3067\u3001\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u30ab\u30a4\u30b3\u306b\u3088\u308b\u6709\u7528\u30bf\u30f3\u30d1\u30af\u8cea\u751f\u7523\u304c\u6ce8\u76ee\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<h3>\u30ab\u30a4\u30b3\u306b\u30ea\u30bd\u30bd\u30fc\u30e0\u75c5\u306e\u6cbb\u7642\u85ac\u3092\u4f5c\u3089\u305b\u308b\u7814\u7a76\u306e\u9014\u4e2d\u7d4c\u904e\uff08\u4f0a\u85e4\u5148\u751f\uff09<\/h3>\n<figure id=\"attachment_4226\" aria-describedby=\"caption-attachment-4226\" style=\"width: 600px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4226\" src=\"https:\/\/dev.academist-cf.com\/journal\/wp-content\/uploads\/2017\/04\/Screen-Shot-2017-04-10-at-12.47.38.png\" alt=\"\" width=\"600\" height=\"452\" srcset=\"https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/04\/Screen-Shot-2017-04-10-at-12.47.38.png 508w, https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/04\/Screen-Shot-2017-04-10-at-12.47.38-300x226.png 300w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption id=\"caption-attachment-4226\" class=\"wp-caption-text\">\u4f0a\u85e4\u5148\u751f<\/figcaption><\/figure>\n<p>\u9175\u7d20\u306e\u5909\u7570\u304c\u539f\u56e0\u3068\u306a\u308a\u3001\u30ea\u30bd\u30bd\u30fc\u30e0\u5185\u3067\u5206\u89e3\u3055\u308c\u308b\u3079\u304d\u751f\u4f53\u7269\u8cea\u304c\u84c4\u7a4d\u3055\u308c\u7570\u5e38\u3092\u304d\u305f\u3059\u306e\u304c\u30ea\u30bd\u30bd\u30fc\u30e0\u75c5\u3067\u3059\u3002\u4eca\u56de\u4f0a\u85e4\u5148\u751f\u306f\u3001\u3053\u306e\u306a\u304b\u3067\u3082\u300c\u30ac\u30e9\u30af\u30c8\u30b7\u30a2\u30ea\u30c9\u30fc\u30b7\u30b9\u300d\u3068 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