{"id":3864,"date":"2017-04-26T10:00:25","date_gmt":"2017-04-26T01:00:25","guid":{"rendered":"https:\/\/dev.academist-cf.com\/journal\/?p=3864"},"modified":"2018-11-01T15:06:57","modified_gmt":"2018-11-01T06:06:57","slug":"%e3%82%b7%e3%83%ad%e3%82%a4%e3%83%8c%e3%83%8a%e3%82%ba%e3%83%8a%e3%81%a7%e9%ab%98%e5%8a%b9%e7%8e%87%e3%81%ae%e3%82%b2%e3%83%8e%e3%83%a0%e7%b7%a8%e9%9b%86%e3%82%92%e5%ae%9f%e7%8f%be%ef%bc%81","status":"publish","type":"post","link":"https:\/\/academist-cf.com\/journal\/?p=3864","title":{"rendered":"\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u3067\u9ad8\u52b9\u7387\u306e\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u5b9f\u73fe\uff01 &#8211; 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id=\"attachment_9016\" aria-describedby=\"caption-attachment-9016\" style=\"width: 600px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-9016 size-full\" src=\"https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/04\/002-15.jpg\" alt=\"\" width=\"600\" height=\"548\" srcset=\"https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/04\/002-15.jpg 600w, https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/04\/002-15-300x274.jpg 300w, https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/04\/002-15-164x150.jpg 164w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption id=\"caption-attachment-9016\" class=\"wp-caption-text\">\u3059\u3079\u3066\u306e\u82b1\u304c\u516b\u91cd\u54b2\u306b\u5909\u5316\u3057\uff08A\uff09\u3001\u82b1\u304b\u3089\u82b1\u304c\u73fe\u308c\u308b\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\uff08B\uff09\u3002\u30b9\u30b1\u30fc\u30eb\u306f5 cm\uff08A\uff09\u30681 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https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/03\/fig3-300x226.jpg 300w, https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/03\/fig3-768x578.jpg 768w, https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/03\/fig3.jpg 1360w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption id=\"caption-attachment-4184\" class=\"wp-caption-text\">\u5168\u8eab\u304c\u30a2\u30eb\u30d3\u30ce\u306b\u5909\u5316\u3057\u305f\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u3002\u63d0\u4f9b\uff1a\u7b52\u4e95\u5927\u8cb4\u535a\u58eb\uff08\u540d\u53e4\u5c4b\u5927\u5b66\uff09<\/figcaption><\/figure>\n<p>\u3055\u3089\u306b\u3001\u809d\u5fc3\u306e\u6b21\u4e16\u4ee3\u304c\u3069\u306e\u304f\u3089\u3044\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u500b\u4f53\u306b\u306a\u308b\u304b\u3092\u8abf\u3079\u305f\u3068\u3053\u308d\u3001\u8abf\u3079\u305f24\u682a\u306e\u3046\u30615\u682a\u306e\u6b21\u4e16\u4ee3\uff08\u81ea\u5bb6\u53d7\u7c89\u306b\u3088\u308a\u5f97\u3089\u308c\u305f\u7a2e\u5b50\uff09\u306b\u304a\u3044\u3066\u3001\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u7a2e\u5b50\u306e\u5272\u5408\u304c100\uff05\uff08\u5e73\u574771\uff05\uff09\u306b\u9054\u3057\u307e\u3057\u305f\u3002\u904e\u53bb\u306e\u5831\u544a\u4f8b\u3067\u306f\u9ad8\u3005\u5e73\u574715\uff05\u7a0b\u5ea6\u3060\u3063\u305f\u3053\u3068\u3092\u8003\u3048\u307e\u3059\u3068\u3001\u5927\u5e45\u306a\u52b9\u7387\u306e\u4e0a\u6607\u3067\u3059\u3002\u305d\u306e\u4ed6\u3001\u3055\u307e\u3056\u307e\u306a\u5de5\u592b\u3092\u65bd\u3059\u3053\u3068\u3067\u3001\u7c21\u4fbf\u304b\u3064\u9ad8\u52b9\u7387\u306b\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u3067\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u884c\u3046\u30d9\u30af\u30bf\u30fc\u3001\u30ab\u30de\u30a4\u30bf\u30c1\uff08pKAMA-ITACHI\uff09\u304c\u8a95\u751f\u3057\u307e\u3057\u305f\u3002\u30ab\u30de\u30a4\u30bf\u30c1\u30fb\u30d9\u30af\u30bf\u30fc\u3092\u7528\u3044\u308b\u3068\u3001\u6a19\u7684\u914d\u5217\u306e\u9078\u5b9a\u304b\u3089\u4e00\u5ea6\u3082PCR\u3092\u7528\u3044\u308b\u3053\u3068\u306a\u304f\u3001\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u7a2e\u5b50\u3092\u53d6\u5f97\u3067\u304d\u307e\u3059\u3002\u3057\u304b\u3082\u30b2\u30ce\u30e0\u7de8\u96c6\u7528\u306e\u907a\u4f1d\u5b50\u304c\u3001\u5206\u96e2\u306b\u3088\u308a\u9664\u304b\u308c\u305f\u7a2e\u5b50\u3060\u3051\u3092\u56de\u53ce\u3067\u304d\u307e\u3059\u3002<\/p>\n<figure id=\"attachment_4186\" aria-describedby=\"caption-attachment-4186\" style=\"width: 600px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4186\" src=\"https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/03\/fig4-1024x779.jpg\" alt=\"\" width=\"600\" height=\"457\" srcset=\"https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/03\/fig4-1024x779.jpg 1024w, https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/03\/fig4-300x228.jpg 300w, https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/03\/fig4-768x584.jpg 768w, https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2017\/03\/fig4.jpg 1255w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption id=\"caption-attachment-4186\" class=\"wp-caption-text\">pKAMA-ITACHI (pKIR)\u30d9\u30af\u30bf\u30fc\u3092\u7528\u3044\u305f\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u6d41\u308c<\/figcaption><\/figure>\n<p>\u30ab\u30de\u30a4\u30bf\u30c1\u306f\u3001\u722a\u304c\u938c\u306b\u306a\u3063\u3066\u3044\u308b\u3001\u30a4\u30bf\u30c1\u306e\u3088\u3046\u306a\u65e5\u672c\u306e\u5996\u602a\u3067\u3059\u3002\u4e2d\u90e8\u5730\u65b9\u306b\u4f1d\u308f\u308b\u30ab\u30de\u30a4\u30bf\u30c1\u306f\u3001\u4e09\u5339\u304c\u4e00\u7dd2\u306b\u50cd\u304d\u307e\u3059\u3002\u4eba\u3092\u8ee2\u3070\u305b\uff08\u4e00\u5339\u76ee\uff09\u3001\u5207\u308a\u3064\u3051\uff08\u4e8c\u5339\u76ee\uff09\u3001\u85ac\u3092\u3064\u3051\u3066\u6cbb\u3059\uff08\u4e09\u5339\u76ee\uff09\u3068\u3044\u3046\u4e00\u9023\u306e\u6d41\u308c\u304c\u3001CRISPR\/Cas9\u3092\u9023\u60f3\u3055\u305b\u308b\u305f\u3081\u3001\u30ab\u30de\u30a4\u30bf\u30c1\u30fb\u30d9\u30af\u30bf\u30fc\u3068\u540d\u4ed8\u3051\u307e\u3057\u305f\u3002<\/p>\n<h3>\u30ab\u30de\u30a4\u30bf\u30c1\u30fb\u30d9\u30af\u30bf\u30fc\u3067\u4f55\u304c\u3067\u304d\u308b\u306e\uff1f<\/h3>\n<p>\u30e2\u30c7\u30eb\u690d\u7269\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u3067\u9ad8\u52b9\u7387\u306a\u65b9\u6cd5\u304c\u958b\u767a\u3055\u308c\u305f\u305f\u3081\u3001\u307e\u305a\u690d\u7269\u79d1\u5b66\u304c\u5927\u304d\u304f\u9032\u5c55\u3059\u308b\u3068\u671f\u5f85\u3055\u308c\u307e\u3059\u3002\u305f\u3068\u3048\u3070\u3001\u690d\u7269\u3067\u7d30\u80de\u9593\u306e\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u306b\u91cd\u8981\u306a\u50cd\u304d\u3092\u3059\u308b\u3053\u3068\u304c\u6b21\u3005\u306b\u660e\u3089\u304b\u306b\u306a\u3063\u3066\u3044\u308b\u5c0f\u3055\u306a\u30bf\u30f3\u30d1\u30af\u8cea\uff08\u30da\u30d7\u30c1\u30c9\uff09\u3092\u30b3\u30fc\u30c9\u3059\u308b\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u89e3\u6790\u304c\u9032\u307f\u307e\u3059\u3002\u907a\u4f1d\u5b50\u3068\u3057\u3066\u3082\u5c0f\u3055\u304f\u3001\u591a\u91cd\u907a\u4f1d\u5b50\u3067\u3001\u30b2\u30ce\u30e0\u4e0a\u306e\u8fd1\u3044\u4f4d\u7f6e\u306b\u4f3c\u305f\u907a\u4f1d\u5b50\u304c\u4e26\u3093\u3067\u3044\u308b\u4f8b\u3082\u591a\u304f\u3001\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u306b\u304a\u3044\u3066\u52b9\u7387\u306e\u3088\u3044\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u304c\u306a\u3051\u308c\u3070\u9032\u5c55\u3057\u306a\u3044\u5206\u91ce\u306e\u3072\u3068\u3064\u3067\u3059\u3002<\/p>\n<p>\u305d\u3057\u3066\u3001\u30ab\u30de\u30a4\u30bf\u30c1\u30fb\u30d9\u30af\u30bf\u30fc\u306e\u958b\u767a\u306f\u3001\u751f\u6b96\u7d30\u80de\u3078\u306e\u907a\u4f1d\u5b50\u5c0e\u5165\u306b\u3088\u308b\u9ad8\u52b9\u7387\u306a\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u53ef\u80fd\u306b\u3057\u307e\u3059\u3002RPS5A\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306f\u4ed6\u690d\u7269\u306e\u751f\u6b96\u7d30\u80de\u3067\u3082\u50cd\u304d\u307e\u3059\u3002\u307e\u3060\u751f\u6b96\u7d30\u80de\u3078\u5b89\u5b9a\u3057\u3066\u5916\u6765\u907a\u4f1d\u5b50\u3092\u5c0e\u5165\u3067\u304d\u308b\u306e\u306f\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u3084\u3001\u8fd1\u7e01\u306e\u897f\u6d0b\u30a2\u30d6\u30e9\u30ca\u3068\u3044\u3046\u4f5c\u7269\u3060\u3051\u3067\u3059\u304c\u3001\u751f\u6b96\u7d30\u80de\u3078\u306e\u907a\u4f1d\u5b50\u5c0e\u5165\u6cd5\u306e\u958b\u767a\u306b\u81f3\u308c\u3070\u3001\u4ed6\u306e\u690d\u7269\u3067\u3082\u30ab\u30de\u30a4\u30bf\u30c1\u30fb\u30d9\u30af\u30bf\u30fc\u306f\u5927\u304d\u306a\u5a01\u529b\u3092\u767a\u63ee\u3059\u308b\u3082\u306e\u3068\u671f\u5f85\u3057\u307e\u3059\u3002\u52d5\u7269\u3068\u9055\u3063\u3066\u3001\u690d\u7269\u3067\u306f\u307e\u3060\u4e00\u822c\u306b\u751f\u6b96\u7d30\u80de\u306e\u81ea\u5728\u306a\u64cd\u4f5c\u306f\u3067\u304d\u307e\u305b\u3093\u3002\u690d\u7269\u306b\u304a\u3051\u308b\u751f\u6b96\u79d1\u5b66\u306e\u3055\u3089\u306a\u308b\u767a\u5c55\u3092\u3001\u30ab\u30de\u30a4\u30bf\u30c1\u306f\u864e\u8996\u7708\u3005\u3068\u5f85\u3061\u308f\u3073\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u672c\u7814\u7a76\u306f\u5f53\u7814\u7a76\u5ba4\u306e\u7b52\u4e95\u5927\u8cb4\u6c0f\u3092\u4e2d\u5fc3\u306b\u9032\u3081\u3089\u308c\u307e\u3057\u305f\u3002<\/p>\n<p><strong>\u53c2\u8003\u6587\u732e<\/strong><\/p>\n<p>Tsutsui H, Higashiyama T. pKAMA-ITACHI Vectors for Highly Ef\ufb01cient CRISPR\/Cas9-Mediated Gene Knockout in<em>Arabidopsis thaliana<\/em>. Plant Cell Physiol (2017) 58: 46\u201356.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u79c1\u305f\u3061\u306e\u7814\u7a76\u5ba4\u3067\u306f\u3001\u690d\u7269\u7814\u7a76\u306b\u6700\u3082\u3088\u304f\u4f7f\u308f\u308c\u308b\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u3068\u3044\u3046\u30e2\u30c7\u30eb\u690d\u7269\u3067\u3001\u975e\u5e38\u306b\u9ad8\u3044\u52b9\u7387\u3067\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u884c\u3046\u5206\u5b50\u30c4\u30fc\u30eb\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002\u305d\u306e\u540d\u3082\u30ab\u30de\u30a4\u30bf\u30c1\u30fb\u30d9\u30af\u30bf\u30fc\u3002\u30d9\u30af\u30bf\u30fc\u3068\u306f\u5c0f\u3055\u3044DNA\u65ad\u7247\u3067\u3001 [&hellip;]<\/p>\n","protected":false},"author":120,"featured_media":4184,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[3],"tags":[16,42,15],"class_list":["post-3864","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-outcome","tag-16","tag-42","tag-biology","has_thumb"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/posts\/3864","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/users\/120"}],"replies":[{"embeddable":true,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3864"}],"version-history":[{"count":6,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/posts\/3864\/revisions"}],"predecessor-version":[{"id":9023,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/posts\/3864\/revisions\/9023"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/media\/4184"}],"wp:attachment":[{"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3864"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3864"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3864"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}