{"id":2843,"date":"2016-12-14T10:00:02","date_gmt":"2016-12-14T01:00:02","guid":{"rendered":"https:\/\/dev.academist-cf.com\/journal\/?p=2843"},"modified":"2017-05-01T13:03:42","modified_gmt":"2017-05-01T04:03:42","slug":"%e9%81%ba%e4%bc%9d%e5%ad%90%e6%94%b9%e5%a4%89%e3%83%96%e3%82%bf%e3%81%ae%e4%bd%9c%e6%88%90%e3%81%ab%e6%88%90%e5%8a%9f%ef%bc%81-%e3%81%9d%e3%81%ae%e3%83%9d%e3%82%a4%e3%83%b3%e3%83%88%e3%81%af","status":"publish","type":"post","link":"https:\/\/academist-cf.com\/journal\/?p=2843","title":{"rendered":"\u907a\u4f1d\u5b50\u6539\u5909\u30d6\u30bf\u306e\u4f5c\u6210\u306b\u6210\u529f\uff01 \b- \u305d\u306e\u30dd\u30a4\u30f3\u30c8\u306f\u300c\u96fb\u6c17\u300d\u306e\u529b\u306b"},"content":{"rendered":"

 <\/p>\n

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\u907a\u4f1d\u5b50\u6539\u5909\u30d6\u30bf\u306e\u4f5c\u308a\u304b\u305f<\/h3>\n

\u30d6\u30bf\u306eES\u7d30\u80de\u306f\u672a\u3060\u6a39\u7acb\u3055\u308c\u3066\u3044\u306a\u3044\u305f\u3081\u3001\u907a\u4f1d\u5b50\u6539\u5909\u30d6\u30bf\u306f\u300c\u4f53\u7d30\u80de\u30af\u30ed\u30fc\u30f3\u6cd5\u300d\u3067\u4f5c\u88fd\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u3002\u4f53\u7d30\u80de\u30af\u30ed\u30fc\u30f3\u6cd5\u3067\u306f\u307e\u305a\u3001\u30d6\u30bf\u306e\u4f53\u7d30\u80de\uff08\u76ae\u819a\u306e\u7d30\u80de\u306a\u3069\uff09\u3067\u76ee\u7684\u306e\u907a\u4f1d\u5b50\u3092\u7d44\u63db\u3048\u307e\u3059\uff08STEP.1\uff09\u3002\u3057\u304b\u3057\u3001\u30d6\u30bf\u304b\u3089\u4f53\u7d30\u80de\u3092\u53d6\u308a\u51fa\u3057\u3066\u9577\u671f\u57f9\u990a\u3059\u308b\u306e\u306f\u5927\u5909\u3067\u3001\u4f53\u7d30\u80de\u3067\u306f\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u306e\u52b9\u7387\u304c\u60aa\u3044\u305f\u3081\u3001\u3046\u307e\u304f\u76ee\u7684\u907a\u4f1d\u5b50\u3092\u64cd\u4f5c\u3057\u305f\u7d30\u80de\u3092\u5f97\u308b\u3053\u3068\u304c\u56f0\u96e3\u3067\u3059\u3002\u76ee\u7684\u306e\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u304c\u3067\u304d\u305f\u5f8c\u306f\u3001\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u3092\u3057\u305f\u4f53\u7d30\u80de\u3068\u3001\u6838\u3092\u3042\u3089\u304b\u3058\u3081\u9664\u3044\u305f\u5375\u7d30\u80de\u3092\u878d\u5408\u3055\u305b\u308b\uff08STEP.2\uff09\u306e\u3067\u3059\u304c\u3001\u3053\u306e\u64cd\u4f5c\u306f\u3001\u308f\u305a\u304b0.1mm\u306e\u5375\u7d30\u80de\u306e\u306a\u304b\u306b\u3042\u308b\u6838\u3092\u53d6\u308a\u51fa\u3059\u3068\u3044\u3046\u6280\u8853\u304c\u5fc5\u8981\u306a\u4e0a\u306b\u3001\u878d\u5408\u3055\u305b\u305f\u3042\u3068\u306e\u767a\u751f\u7387\u304c\u975e\u5e38\u306b\u4f4e\u3044\u3068\u3044\u3046\u554f\u984c\u70b9\u304c\u3042\u308a\u307e\u3057\u305f\u3002<\/p>\n

2013\u5e74\u306b\u5831\u544a\u3055\u308c\u305f\u300cCRISPR\/Cas9\u30b7\u30b9\u30c6\u30e0\u300d\u306f\u3001\u753b\u671f\u7684\u306a\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u3067\u3059\u3002\u907a\u4f1d\u60c5\u5831\u3067\u3042\u308b\u30b2\u30ce\u30e0DNA\u3092\u5207\u65ad\u3059\u308b\u9175\u7d20Cas9\u3068\u3001\u3069\u306e\u907a\u4f1d\u60c5\u5831\u3092\u5207\u65ad\u3059\u308b\u304b\u3092\u6c7a\u5b9a\u3059\u308b\u30ac\u30a4\u30c9RNA\u3092\u7d30\u80de\u5185\u306b\u4f9b\u7d66\u3059\u308b\u3053\u3068\u3067\u3001\u76ee\u7684\u907a\u4f1d\u5b50\u306e\u7834\u58ca\u3084\u3001\u907a\u4f1d\u5b50\u914d\u5217\u306e\u4efb\u610f\u306a\u7f6e\u63db\u3001\u5916\u6765\u306e\u907a\u4f1d\u60c5\u5831\u3092\u633f\u5165\u3057\u305f\u7d30\u80de\u3092\u4f5c\u308a\u51fa\u3059\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u6280\u8853\u3092\u5229\u7528\u3057\u3066\u3001\u907a\u4f1d\u5b50\u64cd\u4f5c\u3092\u3057\u305f\u3055\u307e\u3056\u307e\u306a\u57f9\u990a\u7d30\u80de\u3084\u52d5\u7269\u304c\u3064\u304f\u3089\u308c\u3066\u304d\u307e\u3057\u305f\u3002<\/p>\n

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\"fig1\"
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\u30de\u30a6\u30b9\u3067\u306e\u907a\u4f1d\u5b50\u6539\u5909\u64cd\u4f5c<\/h3>\n

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\"fig2\"
\u53d7\u7cbe\u5375\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5\u306b\u3088\u308b\u30de\u30a4\u30aa\u30b9\u30bf\u30c1\u30f3\u907a\u4f1d\u5b50\u6539\u5909\u30d6\u30bf\uff1a\u4e0a\u6bb5\u304c\u4e00\u822c\u7684\u306a\u30d6\u30bf\u3001\u4e0b\u6bb5\u304c\u30de\u30a4\u30aa\u30b9\u30bf\u30c1\u30f3\u907a\u4f1d\u5b50\u6539\u5909\u30d6\u30bf\u3092\u793a\u3059<\/figcaption><\/figure>\n

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\u53c2\u8003\u6587\u732e<\/strong><\/p>\n

    \n
  1. Tanihara F, Takemoto T, Kitagawa E, Rao S, Do L, Onishi A, Yamashita Y, Kosugi C, Suzuki H, Sembon S, Suzuki S, Nakai M, Hashimoto M, Yasue A, Matsuhisa M, Noji N, Fujimura T, Fuchimoto Di, Otoi T. \u00a0Somatic cell reprogramming-free generation of genetically modified pigs Science Advances 2 (9) e1600803 (2016)<\/li>\n
  2. Hashimoto M, Yamashita Y, Takemoto T. Electroporation of Cas9 protein\/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse. Developmental Biology 418 (1): 1-9 (2016)<\/li>\n
  3. Hashimoto M, Takemoto T. Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR\/Cas9-based genome editing. Scientific Reports 5: 11315 (2015)<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

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