{"id":2731,"date":"2016-12-02T10:00:20","date_gmt":"2016-12-02T01:00:20","guid":{"rendered":"https:\/\/dev.academist-cf.com\/journal\/?p=2731"},"modified":"2016-12-17T16:45:20","modified_gmt":"2016-12-17T07:45:20","slug":"%e3%82%b2%e3%83%8e%e3%83%a0%e7%b7%a8%e9%9b%86%e6%8a%80%e8%a1%93%e3%81%a7%e3%80%81%e7%94%9f%e3%81%8d%e3%81%9f%e3%81%be%e3%81%be%e3%81%ae%e6%a4%8d%e7%89%a9%e3%81%ae%e3%82%af%e3%83%ad%e3%83%9e%e3%83%81","status":"publish","type":"post","link":"https:\/\/academist-cf.com\/journal\/?p=2731","title":{"rendered":"\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u3067\u3001\u751f\u304d\u305f\u307e\u307e\u306e\u690d\u7269\u306e\u30af\u30ed\u30de\u30c1\u30f3\u3092\u898b\u308b"},"content":{"rendered":"

 <\/p>\n

\u7d30\u80de\u6838\u5185\u306e\u30af\u30ed\u30de\u30c1\u30f3\u914d\u7f6e<\/h3>\n

\u771f\u6838\u7d30\u80de\u3067\u306f\u3001\u907a\u4f1d\u60c5\u5831\u3092\u6301\u3064DNA\u304c\u5869\u57fa\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3042\u308b\u30d2\u30b9\u30c8\u30f3\u516b\u91cf\u4f53\u306b\u5dfb\u304d\u4ed8\u304d\u3001\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u69cb\u9020\u3092\u53d6\u3063\u3066\u3044\u307e\u3059\u3002\u3055\u3089\u306b\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306f\u51dd\u7e2e\u3057\u3001\u30af\u30ed\u30de\u30c1\u30f3\u3068\u547c\u3070\u308c\u308b\u9ad8\u6b21\u69cb\u9020\u3092\u5f62\u6210\u3057\u3066\u3001\u6838\u5185\u306b\u30b3\u30f3\u30d1\u30af\u30c8\u306b\u6298\u308a\u305f\u305f\u307e\u3063\u3066\u3044\u307e\u3059\u3002\u6838\u5185\u3067\u30af\u30ed\u30de\u30c1\u30f3\u306f\u3001\u305f\u3060\u30e9\u30f3\u30c0\u30e0\u306b\u53ce\u7d0d\u3055\u308c\u3066\u3044\u308b\u308f\u3051\u3067\u306f\u306a\u304f\u3001\u7279\u5b9a\u306e\u914d\u7f6e\u3092\u53d6\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u3066\u3044\u307e\u3059\u3002\u30af\u30ed\u30de\u30c1\u30f3\u306e\u914d\u7f6e\u306e\u5909\u5316\u3068\u3057\u3066\u306f\u3001\u7d30\u80de\u5206\u88c2\u6642\u306b\u304a\u3051\u308b\u51dd\u7e2e\u3001\u5a18\u67d3\u8272\u4f53\u5206\u96e2\u3001\u8131\u51dd\u7e2e\u306b\u4f34\u3046\u5927\u304d\u306a\u52d5\u304d\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u3055\u3089\u306b\u6700\u8fd1\u3067\u306f\u3001\u74b0\u5883\u30b9\u30c8\u30ec\u30b9\u306b\u3088\u308b\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u5236\u5fa1\u3068\u30af\u30ed\u30de\u30c1\u30f3\u914d\u7f6e\u5909\u5316\u3082\u95a2\u9023\u3057\u3066\u3044\u308b\u3089\u3057\u3044\u3001\u3068\u3044\u3063\u305f\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u308a\u3064\u3064\u3042\u308a\u307e\u3059\u3002\u690d\u7269\u306b\u304a\u3044\u3066\u3082\u907a\u4f1d\u5b50\u767a\u73fe\u91cf\u3084DNA\u640d\u50b7\u30b9\u30c8\u30ec\u30b9\u3068\u30af\u30ed\u30de\u30c1\u30f3\u306e\u914d\u7f6e\u306e\u95a2\u4fc2\u3084\u3001\u4f4e\u6e29\u3084\u5149\u306b\u5fdc\u7b54\u3057\u305f\u305d\u308c\u305e\u308c\u95a2\u9023\u3059\u308b\u907a\u4f1d\u5b50\u9818\u57df\u306e\u6838\u5185\u306e\u914d\u7f6e\u306e\u5909\u5316\u304c\u660e\u3089\u304b\u306b\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u3002<\/p>\n

\u3053\u306e\u3088\u3046\u306a\u6838\u5185\u306e\u30af\u30ed\u30de\u30c1\u30f3\u914d\u7f6e\u3092\u89e3\u6790\u3059\u308b\u305f\u3081\u306b\u3001\u3053\u308c\u307e\u3067\u4e3b\u306b\u86cd\u5149in situ\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\uff08FISH\uff09\u6cd5\u304c\u4f7f\u308f\u308c\u3066\u304d\u307e\u3057\u305f\u3002FISH\u6cd5\u306f\u3001\u30e9\u30d9\u30eb\u3057\u305f\u6838\u9178\u30d7\u30ed\u30fc\u30d6\u3092\u7528\u3044\u3001\u30bf\u30fc\u30b2\u30c3\u30c8DNA\u3092\u76f8\u88dc\u7684\u306a\u914d\u5217\u3068\u63a5\u5408\u3055\u305b\u308b\u3053\u3068\uff08\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\uff09\u3067\u30b2\u30ce\u30e0\u4e2d\u306e\u4efb\u610f\u306e\u914d\u5217\u3092\u53ef\u8996\u5316\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u5f37\u529b\u306a\u65b9\u6cd5\u3067\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u7d30\u80de\u306e\u56fa\u5b9a\u3001\u30d7\u30ed\u30fc\u30d6\u3068\u306e\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\u306e\u305f\u3081\u306e\u9ad8\u6e29\u306b\u3088\u308b\u5909\u6027\u7b49\u306e\u51e6\u7406\u304c\u5fc5\u8981\u306a\u305f\u3081\u3001\u751f\u7d30\u80de\u3067\u306e\u89b3\u5bdf\u306b\u5411\u3044\u3066\u304a\u3089\u305a\u3001\u7d4c\u6642\u7684\u306a\u89e3\u6790\u304c\u96e3\u3057\u3044\u306a\u3069\u306e\u554f\u984c\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u690d\u7269\u3067\u306f\u3001\u56fa\u3044\u7d30\u80de\u58c1\u306e\u5b58\u5728\u306e\u305f\u3081\u306b\u9175\u7d20\u51e6\u7406\u306b\u3088\u308a\u7d30\u80de\u3092\u30d0\u30e9\u30d0\u30e9\u306b\u3059\u308b\u3001\u3042\u308b\u3044\u306f\u6838\u3092\u5358\u96e2\u3059\u308b\u306a\u3069\u5f37\u529b\u306a\u51e6\u7406\u304c\u5fc5\u8981\u3067\u3001\u7d44\u7e54\u306e\u69cb\u9020\u3092\u4fdd\u3063\u305f\u307e\u307e\u3067\u306e\u89e3\u6790\u304c\u56f0\u96e3\u3067\u3057\u305f\u3002<\/p>\n

\"%e5%9b%b3%ef%bc%91\"
FISH\u6cd5\u306b\u3088\u308b\u6838\u5185\u30af\u30ed\u30de\u30c1\u30f3\u914d\u7f6e\u306e\u89e3\u6790\u3002\uff08\u5de6\uff09FISH\u6cd5\u306e\u539f\u7406\u3002\uff08\u53f3\uff09\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u306e\u30af\u30ed\u30de\u30c1\u30f3\u914d\u7f6e\u3002\u7dd1\u8272\u306f\u30bb\u30f3\u30c8\u30ed\u30e1\u30a2\u3001\u8d64\u8272\u306f\u30c6\u30ed\u30e1\u30a2\u3001\u9752\u8272\u306f\u6838\u3092\u793a\u3059<\/figcaption><\/figure>\n

\u690d\u7269\u306e\u30af\u30ed\u30de\u30c1\u30f3\u306e\u52d5\u304d\u3092\u898b\u3066\u307f\u305f\u3044<\/h3>\n

\u79c1\u305f\u3061\u306f\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u3092\u5229\u7528\u3057\u305f\u65b9\u6cd5\u3067\u3001\u30e2\u30c7\u30eb\u690d\u7269\u3067\u3042\u308b\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u306e\u30af\u30ed\u30de\u30c1\u30f3\u306e\u751f\u7d30\u80de\u53ef\u8996\u5316\u3092\u8a66\u307f\u307e\u3057\u305f\u3002\u30b2\u30ce\u30e0\u7de8\u96c6\u3068\u306f\u3001\u4efb\u610f\u306e\u6a19\u7684DNA\u914d\u5217\u3092\u7de8\u96c6\u3059\u308b\u6280\u8853\u3067\u3001DNA\u4e8c\u91cd\u9396\u3092\u6838\u9178\u5206\u89e3\u9175\u7d20\u3068\u3044\u3046\u30cf\u30b5\u30df\u3067\u5207\u3063\u3066\u3084\u308a\u3001\u7d30\u80de\u5185\u306eDNA\u4fee\u5fa9\u80fd\u3092\u5229\u7528\u3057\u3066\u3001\u305d\u306e\u90e8\u5206\u306b\u5909\u7570\u3092\u5165\u308c\u305f\u308a\u3001\u3042\u308b\u3044\u306f\u4ed6\u306e\u907a\u4f1d\u5b50\u3092\u5c0e\u5165\u3057\u305f\u308a\u3059\u308b\u6280\u8853\u3067\u3059\u3002<\/p>\n

\"%e5%9b%b3%ef%bc%92\"
TALE-FP\u306b\u3088\u308b\u30af\u30ed\u30de\u30c1\u30f3\u53ef\u8996\u5316\u306e\u539f\u7406\uff08\u4e0a\uff09TALEN\u3068TALE-FP\u306e\u539f\u7406<\/figcaption><\/figure>\n

\u30cf\u30b5\u30df\u3092\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u3044\u3046\u30e9\u30f3\u30d7\u306b\u5909\u3048\u3066\u690d\u7269\u4e2d\u3067\u767a\u73fe\u3055\u305b\u3066\u3084\u308c\u3070\u3001\u4efb\u610f\u306e\u6a19\u7684DNA\u306e\u5834\u6240\u3092\u77e5\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3060\u308d\u3046\u3068\u79c1\u305f\u3061\u306f\u8003\u3048\u307e\u3057\u305f\u3002\u52d5\u7269\u57f9\u990a\u7d30\u80de\u3067\u306f\u3053\u306e\u3088\u3046\u306a\u6280\u8853\u3092\u7528\u3044\u3066\u3001\u65e2\u306b\u30af\u30ed\u30de\u30c1\u30f3\u306e\u53ef\u8996\u5316\u304c\u53ef\u80fd\u3068\u306a\u3063\u3066\u304d\u307e\u3057\u305f\u3002\u4eca\u56de\u79c1\u305f\u3061\u304c\u5229\u7528\u3057\u305f\u65b9\u6cd5\u306f\u3001TALEN\u3068\u3044\u3046\u30b7\u30b9\u30c6\u30e0\u3067\u3059\u3002<\/p>\n

TALEN\u3068\u306f\u3001\u4eba\u5de5DNA\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3042\u308bTAL\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\u3068\u6838\u9178\u5206\u89e3\u9175\u7d20\u3092\u878d\u5408\u3057\u3066\u4f5c\u3089\u308c\u305f\u4eba\u5de5\u6838\u9178\u5206\u89e3\u9175\u7d20\u3067\u3059\u3002TAL\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\u3068\u306f\u3001\u690d\u7269\u75c5\u539f\u7d30\u83cc\u30ad\u30b5\u30f3\u30c8\u30e2\u30ca\u30b9\u5c5e\u304c\u6709\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3001\u5bbf\u4e3b\u306e\u690d\u7269\u7d30\u80de\u5185\u306b\u8f38\u9001\u3055\u308c\u5bbf\u4e3b\u690d\u7269\u30b2\u30ce\u30e0\u4e0a\u306e\u7279\u5b9a\u306eDNA\u914d\u5217\u306b\u7d50\u5408\u3057\u3001\u611f\u67d3\u3057\u305f\u690d\u7269\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u65b9\u6cd5\u3092\u5909\u3048\u3066\u690d\u7269\u75c5\u539f\u7d30\u83cc\u81ea\u3089\u306e\u611f\u67d3\u306b\u6709\u5229\u306a\u74b0\u5883\u3092\u4f5c\u308a\u51fa\u3057\u307e\u3059\u3002TAL\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\u306eDNA\u7d50\u5408\u90e8\u4f4d\u306f\u3001\u7d0434\u30a2\u30df\u30ce\u9178\u304b\u3089\u306a\u308b\u7e70\u308a\u8fd4\u3057\u914d\u5217\u304c\u30bf\u30f3\u30c7\u30e0\u306b\u4e26\u3093\u3060\u30c9\u30e1\u30a4\u30f3\u304b\u3089\u306a\u3063\u3066\u304a\u308a\u3001\u3072\u3068\u3064\u306e\u7e70\u308a\u8fd4\u3057\u914d\u5217\u3067\u3072\u3068\u3064\u306e\u5869\u57fa\u5bfe\u3092\u8a8d\u8b58\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u7e70\u308a\u8fd4\u3057\u914d\u5217\u3092\u7d44\u307f\u5408\u308f\u305b\u308b\u3053\u3068\u3067\u3001\u4efb\u610f\u306eDNA\u914d\u5217\u306b\u7d50\u5408\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u4f5c\u308a\u51fa\u3059\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n

\"%e5%9b%b3%ef%bc%93\"
TALE-FP\u306b\u3088\u308b\u53ef\u8996\u5316\u3002\uff08\u4e0a\uff09\u6839\u7d30\u80de\u306b\u304a\u3051\u308bTALE-FP\u306b\u3088\u308b\u30bb\u30f3\u30c8\u30ed\u30e1\u30a2\u3001\u30c6\u30ed\u30e1\u30a2\u300118S rDNA\u914d\u5217\u306e\u53ef\u8996\u5316\u3002\u9ec4\u8272\u306f\u7d30\u80de\u6838\u3092\u793a\u3059\u3002\uff08\u4e2d\uff09\u8449\u8089\u7d30\u80de\u306b\u304a\u3051\u308bTALE-FP\u306b\u3088\u308b\u30bb\u30f3\u30c8\u30ed\u30e1\u30a2\u306e\u53ef\u8996\u5316\u3002\u9ec4\u8272\u306f\u7d30\u80de\u6838\u3092\u793a\u3059\u3002\uff08\u4e0b\uff09\u6839\u6bdb\u7d30\u80de\u306b\u304a\u3051\u308bTALE-FP\u306b\u3088\u308b\u30c6\u30ed\u30e1\u30a2\u30b7\u30b0\u30ca\u30eb\u306e\u8ffd\u8de1\u3002\u767d\u8272\u306f\u7d30\u80de\u6838\u3092\u793a\u3059\uff08\u53c2\u8003\u6587\u732e\u3088\u308a\u6539\u5909\uff09<\/figcaption><\/figure>\n

\u305f\u3060\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u884c\u3046\u5834\u5408\u3001TALEN\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u6a19\u7684\u90e8\u4f4d\u306b\u4e00\u5bfe\u3042\u308c\u3070DNA\u4e8c\u91cd\u9396\u3092\u5207\u65ad\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u304c\u3001\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u3064\u306a\u3052\u305fTAL\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\uff08TALE-FP\uff09\u3067\u53ef\u8996\u5316\u3059\u308b\u5834\u5408\u3001\u540c\u3058\u4f4d\u7f6e\u306b\u8907\u6570\u306e\u30e9\u30f3\u30d7\u304c\u306a\u3044\u3068\u3001\u7d50\u5408\u3057\u3066\u3044\u306a\u3044TALE-FP\u3042\u308b\u3044\u306f\u690d\u7269\u7531\u6765\u306e\u81ea\u5bb6\u86cd\u5149\u306e\u30ce\u30a4\u30ba\u306b\u57cb\u3082\u308c\u3066\u3057\u307e\u3044\u3001\u30b7\u30b0\u30ca\u30eb\u306e\u691c\u51fa\u304c\u56f0\u96e3\u3067\u3059\u3002\u305d\u3053\u3067\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u306b\u542b\u307e\u308c\u308b\u3044\u304f\u3064\u304b\u306e\u9ad8\u5ea6\u306a\u53cd\u5fa9\u914d\u5217\u3092\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u7528\u3044\u307e\u3057\u305f\u3002<\/p>\n

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\u79c1\u305f\u3061\u306e\u65b9\u6cd5\u3067\u306f\u3001TALE-FP\u306f\u690d\u7269\u4f53\u306e\u81f3\u308b\u7d44\u7e54\u3067\u767a\u73fe\u3057\u3066\u3044\u307e\u3059\u3002\u3072\u3068\u3053\u3068\u3067\u7d30\u80de\u6838\u3068\u3044\u3063\u3066\u3082\u3001\u7d44\u7e54\u3001\u7d30\u80de\u6bce\u306b\u305d\u306e\u59ff\u5f62\u304c\u7570\u306a\u308a\u307e\u3059\u3002\u9855\u5fae\u93e1\u4e0b\u3067\u7d4c\u6642\u89b3\u5bdf\u3092\u3059\u308b\u3068\u30e9\u30d9\u30eb\u3055\u308c\u305f\u9818\u57df\u306f\u6838\u5185\u306e\u7279\u5b9a\u306e\u4f4d\u7f6e\u3067\u7559\u307e\u3063\u3066\u3044\u308b\u306e\u3067\u306f\u306a\u304f\u3001\u7d30\u304b\u304f\u52d5\u304d\u56de\u3063\u3066\u3044\u308b\u3001\u3055\u3089\u306b\u7d30\u80de\u3054\u3068\u306b\u305d\u306e\u52d5\u304d\u306b\u3069\u3046\u3084\u3089\u5dee\u7570\u304c\u3042\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u3066\u304d\u307e\u3057\u305f\u3002\u73fe\u5728\u306e\u3068\u3053\u308d\u3001\u30af\u30ed\u30de\u30c1\u30f3\u306e\u52d5\u304d\u306e\u9055\u3044\u304c\u3069\u306e\u3088\u3046\u306a\u7d30\u80de\u751f\u7406\u3068\u95a2\u4fc2\u304c\u3042\u308b\u306e\u304b\u307e\u3060\u308f\u304b\u3063\u3066\u3044\u307e\u305b\u3093\u3002<\/p>\n

\u4eca\u5f8c\u306e\u5c55\u671b<\/h3>\n

\u307e\u3060\u9ad8\u5ea6\u306a\u53cd\u5fa9\u914d\u5217\u306b\u5bfe\u3057\u3066\u3057\u304b\u53ef\u8996\u5316\u306b\u6210\u529f\u3057\u3066\u3044\u307e\u305b\u3093\u304c\u3001\u4eca\u5f8c\u306f\u3082\u3046\u5c11\u3057\u77ed\u3044\u9818\u57df\u3092\u30e9\u30d9\u30eb\u3059\u308b\u5de5\u592b\u3092\u52a0\u3048\u3066\u3001\u6700\u7d42\u7684\u306b\u4efb\u610f\u306e\u4e00\u907a\u4f1d\u5b50\u9818\u57df\u3067\u306e\u53ef\u8996\u5316\u3059\u308b\u3053\u3068\u3067\u3001\u3055\u3089\u306b\u30af\u30ed\u30de\u30c1\u30f3\u914d\u7f6e\u53ca\u3073\u52d5\u304d\u3068\u3001\u7d30\u80de\u6a5f\u80fd\u3068\u306e\u95a2\u9023\u3092\u898b\u51fa\u3057\u3066\u3044\u3051\u305f\u3089\u3001\u3068\u8003\u3048\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u53c2\u8003\u6587\u732e
\n<\/strong>
\nFujimoto, S., Sugano, S. S., Kuwata, K., Osakabe, K. and Matsunaga, S. (2016) Visualization of specific repetitive genomic sequences with fluorescent TALEs in Arabidopsis thaliana. Journal of Experimental Botany 67, 6101-6110.<\/p>\n","protected":false},"excerpt":{"rendered":"

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