{"id":10809,"date":"2019-05-16T08:00:48","date_gmt":"2019-05-15T23:00:48","guid":{"rendered":"https:\/\/academist-cf.com\/journal\/?p=10809"},"modified":"2019-05-16T10:57:21","modified_gmt":"2019-05-16T01:57:21","slug":"%e9%81%ba%e4%bc%9d%e5%ad%90%e3%81%8c%e8%bb%a2%e5%86%99%e3%81%95%e3%82%8c%e3%82%8b%e3%81%a8%e3%81%8d%e3%81%ae%e3%83%8c%e3%82%af%e3%83%ac%e3%82%aa%e3%82%bd%e3%83%bc%e3%83%a0%e3%81%ae%e5%8b%95%e3%81%8d","status":"publish","type":"post","link":"https:\/\/academist-cf.com\/journal\/?p=10809","title":{"rendered":"\u907a\u4f1d\u5b50\u304c\u8ee2\u5199\u3055\u308c\u308b\u3068\u304d\u306e\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e&#8221;\u52d5\u304d&#8221;\u3092\u6349\u3048\u305f\uff01"},"content":{"rendered":"<h3>\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u3075\u308b\u307e\u3044\u3068\u306f\uff1f<\/h3>\n<p>\u79c1\u305f\u3061\u306e\u4f53\u306f\u7d0440\u5146\u500b\u306e\u7d30\u80de\u304b\u3089\u69cb\u6210\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u308c\u305e\u308c\u306e\u7d30\u80de\u306b\u306f\u3001\u751f\u547d\u306e\u8a2d\u8a08\u56f3\u3068\u3082\u79f0\u3055\u308c\u308b\u5168\u9577\u7d042m\u306e\u30d2\u30c8\u30b2\u30ce\u30e0DNA\u304c\u53ce\u7d0d\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306eDNA\u306f\u76f4\u5f842nm\uff0810<sup>-9<\/sup>m\uff09\u306e\u3068\u3066\u3082\u7d30\u3044\u7cf8\u3067\u3001\u300c\u30d2\u30b9\u30c8\u30f3\u300d\u3068\u3088\u3070\u308c\u308b\u6a3d\u72b6\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u5dfb\u304b\u308c\u308b\u3053\u3068\u3067\u3001\u76f4\u5f84\u7d0411nm\u306e\u300c\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u300d\u3092\u4f5c\u3063\u3066\u3044\u307e\u3059\u3002\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u3068\u3001\u3053\u308c\u306b\u7d50\u5408\u3059\u308b\u4ed6\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5408\u308f\u305b\u3066\u300c\u30af\u30ed\u30de\u30c1\u30f3\u300d\u3068\u547c\u3073\u307e\u3059\u3002<\/p>\n<p>\u9577\u3044\u3042\u3044\u3060\u3001\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306f\u3089\u305b\u3093\u72b6\u306b\u898f\u5247\u6b63\u3057\u304f\u6298\u308a\u305f\u305f\u307e\u308c\uff08\u30af\u30ed\u30de\u30c1\u30f3\u7dda\u7dad\uff09\u3001\u3055\u3089\u306b\u5dfb\u304b\u308c\u3066\u968e\u5c64\u69cb\u9020\u3092\u4f5c\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u304d\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u79c1\u305f\u3061\u306f2007\u5e74\u3088\u308a\u30af\u30e9\u30a4\u30aa\u96fb\u5b50\u9855\u5fae\u93e1\u3084X\u7dda\u6563\u4e71\u3092\u7528\u3044\u305f\u5b9f\u9a13\u306b\u3088\u3063\u3066\u3001\u5b9a\u8aac\u306e\u3088\u3046\u306a\u898f\u5247\u6b63\u3057\u3044\u968e\u5c64\u69cb\u9020\u306f\u5b58\u5728\u305b\u305a\u3001\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u304c\u4e0d\u898f\u5247\u306b\u3001\u305d\u3057\u3066\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u306b\u7d44\u7e54\u5316\u3055\u308c\u3001\u7d30\u80de\u5185\u306b\u53ce\u3081\u3089\u308c\u3066\u3044\u308b\u3053\u3068\u3092\u63d0\u5531\u3057\u3066\u304d\u307e\u3057\u305f\u3002<\/p>\n<figure id=\"attachment_10824\" aria-describedby=\"caption-attachment-10824\" style=\"width: 600px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2019\/05\/001-2.jpg\" alt=\"\" width=\"600\" height=\"674\" class=\"size-full wp-image-10824\" srcset=\"https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2019\/05\/001-2.jpg 600w, https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2019\/05\/001-2-267x300.jpg 267w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption id=\"caption-attachment-10824\" class=\"wp-caption-text\">\uff08\u4e0a\u6bb5\uff09DNA\u306f\u30d2\u30b9\u30c8\u30f3\u306b\u5dfb\u304b\u308c\u3066\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u3092\u5f62\u6210\u3059\u308b\u3002\u3053\u3053\u3067\u306f3\u500b\u306e\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u304c\u63cf\u304b\u308c\u3066\u3044\u308b\u304c\u3001\u7d30\u80de1\u500b\u5f53\u305f\u308a3000\u4e07\u500b\u3082\u306e\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u304c\u5b58\u5728\u3059\u308b\u3002\uff08\u4e2d\u6bb5\uff09DNA\u306e\u7d30\u80de\u5185\u53ce\u7d0d\u306b\u95a2\u3059\u308b\u3053\u308c\u307e\u3067\u306e\u5b9a\u8aac\uff08\u5de6\uff09\u3068\u73fe\u5728\u306e\u8aac\uff08\u53f3\uff09\u3002<\/figcaption><\/figure>\n<p>\u3053\u308c\u4ee5\u964d\u3001\u751f\u304d\u305f\u7d30\u80de\u5185\u3067\u306e\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u3075\u308b\u307e\u3044\u306b\u3064\u3044\u3066\u7406\u89e3\u304c\u6c42\u3081\u3089\u308c\u3066\u304d\u307e\u3057\u305f\u304c\u3001\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306f\u6570\u304c\u81a8\u5927\u3067\u304b\u3064\u305d\u306e\u52d5\u304d\u306f\u975e\u5e38\u306b\u5c0f\u3055\u304f\u3001\u5f93\u6765\u306e\u5149\u5b66\u9855\u5fae\u93e1\u3092\u7528\u3044\u3066\u89b3\u5bdf\u3059\u308b\u3053\u3068\u306f\u56f0\u96e3\u3067\u3057\u305f\u3002<\/p>\n<h3>\u907a\u4f1d\u5b50\u304c\u8ee2\u5199\u3055\u308c\u308b\u3068\u304d\u306e\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u3068\u306f\uff1f<\/h3>\n<p>\u79c1\u305f\u3061\u306e\u7814\u7a76\u5ba4\u3067\u306f\u3001\u8d85\u89e3\u50cf\u86cd\u5149\u9855\u5fae\u93e1\u3092\u99c6\u4f7f\u3059\u308b\u3053\u3068\u3067\u3001\u500b\u3005\u306e\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u3075\u308b\u307e\u3044\u3092\u3001\u751f\u304d\u305f\u307e\u307e\u306e\u7d30\u80de\u5185\u3067\u89b3\u5bdf\u3059\u308b\u300c\u5358\u4e00\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u300d\u306b\u6210\u529f\u3057\u307e\u3057\u305f\u3002<\/p>\n<p>\u6210\u529f\u306e\u9375\u306f\u3075\u305f\u3064\u3042\u308a\u307e\u3057\u305f\u3002\u3072\u3068\u3064\u306f\u3001\u7d30\u80de1\u500b\u3042\u305f\u308a3000\u4e07\u500b\u7a0b\u5ea6\u5b58\u5728\u3059\u308b\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u3092\u3001\u4f4e\u6fc3\u5ea6\u306e\u86cd\u5149\u8272\u7d20\uff08\u30c6\u30c8\u30e9\u30e1\u30c1\u30eb\u30ed\u30fc\u30c0\u30df\u30f3; TMR\uff09\u3067\u307e\u3070\u3089\u306b\u6a19\u8b58\u3057\u3001\u5149\u308b\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u6570\u3092\u5236\u9650\u3057\u305f\u3053\u3068\u3067\u3059\u3002\u3082\u3046\u3072\u3068\u3064\u306f\u3001\u30b7\u30fc\u30c8\u72b6\u306e\u5149\u3067\u7d30\u80de\u6838\u3092\u7167\u3089\u3059\u3053\u3068\u3067\u3001\u5149\u304c\u6a2a\u5207\u308b\u6975\u8584\u3044\u9818\u57df\u306b\u3042\u308b\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u3060\u3051\u3092\u5149\u3089\u305b\u3001\u9855\u5fae\u93e1\u89b3\u5bdf\u306b\u304a\u3051\u308b\u80cc\u666f\u5149\u3092\u5287\u7684\u306b\u6291\u3048\u305f\u3053\u3068\u3067\u3059\u3002\u3053\u308c\u3089\u306e\u5de5\u592b\u306b\u3088\u308a\u3001\u751f\u304d\u305f\u7d30\u80de\u306e\u4e2d\u3067\u500b\u3005\u306e\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u3092\u64ae\u5f71\u3057\u3001\u305d\u306e\u52d5\u304d\u3092\u8a73\u7d30\u306b\u8abf\u3079\u308b\u3053\u3068\u304c\u53ef\u80fd\u3068\u306a\u308a\u307e\u3057\u305f\u3002<\/p>\n<p>\u79c1\u305f\u3061\u306f\u3001\u64ae\u5f71\u3057\u305f\u500b\u3005\u306e\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u8f1d\u70b9\u30922\u6b21\u5143\u30ac\u30a6\u30b9\u95a2\u6570\u3067\u30d5\u30a3\u30c3\u30c8\u3057\u3001\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u4f4d\u7f6e\u3092\u9ad8\u7cbe\u5ea6\uff0815nm\uff09\u3067\u6c7a\u5b9a\u3057\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u3001\u500b\u3005\u306e\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u3092\u8ffd\u8de1\u3059\u308b\u3053\u3068\u3067\u3001\u4e00\u5b9a\u6642\u9593\u306b\u304a\u3051\u308b\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u306e\u5927\u304d\u3055\u306e\u6307\u6a19\u3067\u3042\u308b\u5e73\u5747\u4e8c\u4e57\u5909\u4f4d\u3082\u898b\u7a4d\u3082\u308a\u307e\u3057\u305f\u3002<\/p>\n<p>\u6b21\u306b\u3001\u5358\u4e00\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3092\u7528\u3044\u3066\u3001\u300c\u907a\u4f1d\u5b50\u304c\u8ee2\u5199\u3055\u308c\u308b\u969b\u306e\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u300d\u3092\u89e3\u6790\u3057\u307e\u3057\u305f\u3002\u7d30\u80de\u3092RNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bcII\uff08RNAPII\uff09\u306e\u963b\u5bb3\u5264\u3067\u51e6\u7406\u3057\u3066\u8ee2\u5199\u3092\u963b\u5bb3\u3057\u3001\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u3092\u89e3\u6790\u3057\u305f\u3068\u3053\u308d\u3001\u9a5a\u304f\u3079\u304d\u3053\u3068\u306b\u3001\u305d\u306e\u6295\u4e0e\u91cf\u306b\u4f9d\u5b58\u3057\u3066\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u304c\u6d3b\u767a\u306b\u306a\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u306e\u3067\u3059\u3002<\/p>\n<figure id=\"attachment_10806\" aria-describedby=\"caption-attachment-10806\" style=\"width: 600px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-10806\" src=\"https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2019\/05\/002.jpg\" alt=\"\" width=\"600\" height=\"214\" srcset=\"https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2019\/05\/002.jpg 600w, https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2019\/05\/002-300x107.jpg 300w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption id=\"caption-attachment-10806\" class=\"wp-caption-text\">(A) \u307e\u3070\u3089\u306b\u86cd\u5149\u6a19\u8b58\u3055\u308c\u305f\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\uff08\u8d64\uff09\u3002\u3053\u308c\u3089\u306b\u6ce8\u76ee\u3059\u308b\u3053\u3068\u3067\u500b\u3005\u306e\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u3092\u8abf\u3079\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3002\u30d2\u30b9\u30c8\u30f3H2B\u3092HaloTag\u3068\u878d\u5408\u3057(H2B-Halo)\u3001\u7d30\u80de\u5185\u3067\u767a\u73fe\u3055\u305b\u3001\u3053\u306e\u7d30\u80de\u3092\u86cd\u5149\u8272\u7d20\u30c6\u30c8\u30e9\u30e1\u30c1\u30eb\u30ed\u30fc\u30c0\u30df\u30f3\uff08TMR\uff09\u304c\u4ed8\u52a0\u3055\u308c\u305fHaloTag\u30ea\u30ac\u30f3\u30c9\u3067\u51e6\u7406\u3059\u308b\u3053\u3068\u3067\u3001\u7d30\u80de\u5185\u306e\u3054\u304f\u5c11\u6570\u306eH2B\u3092\u7279\u7570\u7684\u306b\u86cd\u5149\u6a19\u8b58\u3057\u305f\u3002\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u5185\u306eH2B-Halo\u306f\u3042\u308b\u6642\u9593\u3067\u7d76\u3048\u305a\u5165\u308c\u66ff\u308f\u3063\u3066\u3044\u308b\u305f\u3081\u3001\u6642\u9593\u5358\u4f4d\u3067\u30e6\u30fc\u30af\u30ed\u30de\u30c1\u30f3\u3001\u30d8\u30c6\u30ed\u30af\u30ed\u30de\u30c1\u30f3\u306e\u5225\u306a\u304f\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306b\u53d6\u308a\u8fbc\u307e\u308c\u308b\u3002(B) \u659c\u5149\u7167\u660e\u86cd\u5149\u9855\u5fae\u93e1\u6cd5\u306b\u3088\u308b\u751f\u304d\u305f\u7d30\u80de\u306e\u6838\u5185\u306e\u5358\u4e00\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u753b\u50cf\u3002\u3072\u3068\u3064\u306e\u8f1d\u70b9\u304c\u3072\u3068\u3064\u306e\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u3092\u793a\u3057\u3066\u3044\u308b\u3002(C) \u8ee2\u5199\u3092\u963b\u5bb3\u3059\u308b\u3068\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u304c\u4e0a\u6607\u3059\u308b\uff08\u8d64\u7dda\uff09\u3002\u9ed2\u7dda\u304c\u901a\u5e38\u72b6\u614b\u306e\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u3002\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u306f\u5e73\u5747\u4e8c\u4e57\u5909\u4f4d\u3067\u8868\u3055\u308c\u3066\u3044\u308b\u3002\uff08Nagashima <em>et al.<\/em>, 2019\u3088\u308a\uff09<\/figcaption><\/figure>\n<p>\u3053\u3053\u3067\u3001\u89b3\u5bdf\u3057\u305f\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u3092\u52d5\u753b\u3067\u898b\u3066\u307f\u307e\u3057\u3087\u3046\u3002\u5de6\u5074\u306f\u901a\u5e38\u72b6\u614b\u306e\u7d30\u80de\u5185\u306e\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u3067\u3001\u3072\u3068\u3064\u3072\u3068\u3064\u306e\u30c9\u30c3\u30c8\u304c\u3072\u3068\u3064\u3072\u3068\u3064\u306e\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u53f3\u5074\u306f\u8ee2\u5199\u3092\u963b\u5bb3\u3057\u305f\u969b\u306e\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u3067\u3001\u5de6\u5074\u306e\u901a\u5e38\u72b6\u614b\u3068\u6bd4\u3079\u3066\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u304c\u6d3b\u767a\u5316\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\u3002<\/p>\n<p><iframe loading=\"lazy\" src=\"https:\/\/www.youtube.com\/embed\/q8zP5hUfzr8\" width=\"600\" height=\"337\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>\u8d85\u89e3\u50cf\u86cd\u5149\u9855\u5fae\u93e1\u306b\u3088\u308b\u6838\u5185\u306e\u5358\u4e00\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u306e\u52d5\u753b\uff081\u30b3\u30de50\u30df\u30ea\u79d2\uff09\uff08Nagashima <em>et al.<\/em>, 2019\u3088\u308a\uff09<\/p>\n<p>\u8907\u6570\u306e\u8ee2\u5199\u963b\u5bb3\u5264\u3092\u8a66\u3057\u3066\u3082\u3001\u540c\u69d8\u306e\u7d50\u679c\u304c\u5f97\u3089\u308c\u307e\u3057\u305f\u3002\u3064\u307e\u308a\u3001\u8ee2\u5199\u304c\u8d77\u3053\u308b\u969b\u3001\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u304c\u675f\u7e1b\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f\u306e\u3067\u3059\u3002\u4e00\u822c\u306b\u3001\u8ee2\u5199\u3055\u308c\u3066\u3044\u308b\u30af\u30ed\u30de\u30c1\u30f3\u9818\u57df\u306f\u3001\u30aa\u30fc\u30d7\u30f3\u3067\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u3067\u3042\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u304d\u305f\u306e\u3067\u3001\u3053\u306e\u7d50\u679c\u306f\u4e88\u671f\u305b\u306c\u3082\u306e\u3067\u3057\u305f\u3002<\/p>\n<p>\u4e00\u65b9\u3001\u963b\u5bb3\u5264\u51e6\u7406\u306b\u306f\u5e38\u306b\u3055\u307e\u3056\u307e\u306a\u9593\u63a5\u52b9\u679c\u306e\u30ea\u30b9\u30af\u304c\u3064\u304d\u307e\u3068\u3044\u307e\u3059\u3002\u305f\u3068\u3048\u3070\u3001\u3042\u308b\u7a2e\u306e\u8ee2\u5199\u963b\u5bb3\u5264\u51e6\u7406\u3092\u9577\u6642\u9593\u884c\u3046\u3068\u6838\u306e\u5185\u90e8\u69cb\u9020\u304c\u5909\u5316\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u3053\u3067\u3001\u3088\u308a\u76f4\u63a5\u7684\u306b\u300c\u8ee2\u5199\u304c\u963b\u5bb3\u3055\u308c\u305f\u72b6\u614b\u306e\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u300d\u3092\u8a55\u4fa1\u3059\u308b\u305f\u3081\u3001\u56fd\u7acb\u907a\u4f1d\u5b66\u7814\u7a76\u6240\u30fb\u9418\u5dfb\u5c06\u4eba\u6559\u6388\u3089\u304c\u8003\u6848\u3057\u305f\u30aa\u30fc\u30ad\u30b7\u30f3\u30c7\u30b0\u30ed\u30f3\uff08AID\uff09\u6cd5\u3092\u7528\u3044\u3066\u3001RNAPII\u306e\u6700\u3082\u5927\u304d\u306a\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u3067\u3042\u308bRPB1\u3092\u6025\u901f\u306b\u3001\u305d\u3057\u3066\u7279\u7570\u7684\u306b\u5206\u89e3\u9664\u53bb\u3057\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001\u8ee2\u5199\u963b\u5bb3\u5264\u51e6\u7406\u3092\u884c\u3063\u305f\u3068\u304d\u3068\u540c\u69d8\u306b\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u304c\u9855\u8457\u306b\u4e0a\u6607\u3057\u307e\u3057\u305f\u3002\u300c\u8ee2\u5199\u3092\u963b\u5bb3\u3059\u308b\u3068\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u304c\u4e0a\u6607\u3059\u308b\u300d\u3053\u3068\u3078\u306e\u3055\u3089\u306a\u308b\u78ba\u8a3c\u304c\u5f97\u3089\u308c\u305f\u308f\u3051\u3067\u3059\u3002<\/p>\n<h3>\u30af\u30ed\u30de\u30c1\u30f3\u306f\u300c\u8ee2\u5199\u30cf\u30d6\u300d\u3092\u4ecb\u3057\u3066\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u3092\u3064\u304f\u308b<\/h3>\n<p>RNAPII\u306b\u3088\u308b\u8ee2\u5199\u3092\u963b\u5bb3\u3059\u308b\u3068\u3001\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u304c\u4e0a\u6607\u3059\u308b\u3053\u3068\u304b\u3089\u3001RNAPII\u304c\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u3092\u6291\u3048\u3066\u3044\u308b\u3068\u601d\u308f\u308c\u307e\u3059\u3002\u3053\u306e\u3053\u3068\u304b\u3089\u79c1\u305f\u3061\u306f\u3001\u30af\u30ed\u30de\u30c1\u30f3\u306f\u8ee2\u5199\u304c\u8d77\u3053\u308b\u5834\u6240\uff08RNAPII\u306e\u96c6\u307e\u3063\u305f\u3068\u3053\u308d\uff09\u3092\u300c\u30cf\u30d6\u300d\u3068\u3057\u3066\u3001\u3086\u308b\u3084\u304b\u306a\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u3092\u5f62\u6210\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u307e\u3057\u305f\u3002\u3064\u307e\u308a\u3001\u901a\u5e38\u72b6\u614b\u3067\u306f\u3053\u306e\u3086\u308b\u3084\u304b\u306a\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u304c\u30af\u30ed\u30de\u30c1\u30f3\u306e\u52d5\u304d\u3092\u30b0\u30eb\u30fc\u30d0\u30eb\u306b\u6291\u5236\u3057\u3066\u304a\u308a\u3001RNAPII\u3092\u963b\u5bb3\u307e\u305f\u306f\u9664\u53bb\u3059\u308b\u3068\u3001\u3053\u306e\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u304c\u5931\u308f\u308c\u3066\u30af\u30ed\u30de\u30c1\u30f3\u306e\u52d5\u304d\u304c\u4e0a\u6607\u3059\u308b\u308f\u3051\u3067\u3059\u3002<\/p>\n<figure id=\"attachment_10807\" aria-describedby=\"caption-attachment-10807\" style=\"width: 600px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-10807\" src=\"https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2019\/05\/003.jpg\" alt=\"\" width=\"600\" height=\"201\" srcset=\"https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2019\/05\/003.jpg 600w, https:\/\/academist-cf.com\/journal\/wp-content\/uploads\/2019\/05\/003-300x101.jpg 300w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption id=\"caption-attachment-10807\" class=\"wp-caption-text\">\u7d30\u80de\u306e\u6838\uff08\u5de6\uff09\u306e\u4e2d\u3067\u8ee2\u5199\u304c\u8d77\u304d\u308b\u969b\u3001\u30af\u30ed\u30de\u30c1\u30f3\u4e0a\u3067\u50cd\u304fRNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bcII\uff08\u8d64\uff09\u3084\u4ed6\u306e\u8ee2\u5199\u56e0\u5b50\uff08\u9752\uff09\u304c\u30cf\u30d6\uff08\u30d4\u30f3\u30af\u8272\uff09\u3092\u4f5c\u3063\u3066\u30af\u30ed\u30de\u30c1\u30f3\u306e\u52d5\u304d\u3092\u6291\u3048\u308b\uff08\u4e2d\u592e\uff09\u3002\u7d30\u80de\u6838\u5185\u306e\u30af\u30ed\u30de\u30c1\u30f3\u306f\u8ee2\u5199\u304c\u8d77\u304d\u308b\u5834\u6240\u3092\u30cf\u30d6\u3068\u3057\u3001\u3086\u308b\u3084\u304b\u306a\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u3092\u4f5c\u3063\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u3002\u8ee2\u5199\u3092\u963b\u5bb3\u3059\u308b\u3068\u30af\u30ed\u30de\u30c1\u30f3\u3092\u62d8\u675f\u3057\u3066\u3044\u308b\u30cf\u30d6\u304c\u7121\u304f\u306a\u308a\u3001\u30af\u30ed\u30de\u30c1\u30f3\u306e\u52d5\u304d\u304c\u4e0a\u6607\u3059\u308b\uff08\u53f3\uff09\u3002\uff08Nagashima <em>et al.<\/em>, 2019\u3088\u308a\uff09<\/figcaption><\/figure>\n<p>\u3053\u306e\u8003\u3048\u3092\u8a66\u3059\u305f\u3081\u306b\u3001\u540d\u53e4\u5c4b\u5927\u5b66\u5de5\u5b66\u7814\u7a76\u79d1\u30fb\u7b39\u4e95\u7406\u751f\u6559\u6388\u30b0\u30eb\u30fc\u30d7\u306e\u5354\u529b\u3092\u5f97\u3066\u3001\u8a08\u7b97\u6a5f\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u306b\u3088\u3063\u3066\u30af\u30ed\u30de\u30c1\u30f3\u74b0\u5883\u3092\u518d\u69cb\u7bc9\u3057\u307e\u3057\u305f\u3002RNAPII\u306e\u8ee2\u5199\u30cf\u30d6\u306e\u3042\u308a\u306a\u3057\u3067\u3001\u30af\u30ed\u30de\u30c1\u30f3\u306e\u52d5\u304d\u3092\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u3057\u305f\u7d50\u679c\u3001\u4e88\u6e2c\u901a\u308aRNAPII\u306e\u30cf\u30d6\u3092\u9664\u304f\u3068\u30af\u30ed\u30de\u30c1\u30f3\u306e\u52d5\u304d\u306f\u4e0a\u6607\u3057\u307e\u3057\u305f\u3002<\/p>\n<p>\u305d\u306e\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u7d50\u679c\u3092\u52d5\u753b\u3067\u898b\u3066\u307f\u307e\u3057\u3087\u3046\u3002\u7dd1\u306e\u30dc\u30fc\u30eb\u306f\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u584a\u3001\u30d4\u30f3\u30af\u306e\u30dc\u30fc\u30eb\u306f\u8ee2\u5199\u30cf\u30d6\u3001\u8d64\u3044\u30dc\u30fc\u30eb\u306f\u8ee2\u5199\u3057\u3066\u3044\u308bRNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bcII\u3092\u8868\u3057\u3066\u3044\u307e\u3059\u3002\u8ee2\u5199\u30cf\u30d6\u304c\u5b58\u5728\u3057\u306a\u3044\u5834\u5408\u306f\u3001\u8ee2\u5199\u304c\u963b\u5bb3\u3055\u308c\u305f\u72b6\u6cc1\u3092\u793a\u3057\u3066\u304a\u308a\u3001\u8ee2\u5199\u30cf\u30d6\u304c\u5b58\u5728\u3059\u308b\u72b6\u6cc1\u3088\u308a\u7dd1\u306e\u30dc\u30fc\u30eb\u304c\u6fc0\u3057\u304f\u52d5\u3044\u3066\u3044\u308b\u306e\u304c\u308f\u304b\u308a\u307e\u3059\u3002<\/p>\n<p><iframe loading=\"lazy\" src=\"https:\/\/www.youtube.com\/embed\/LkkYIAz5I9U\" width=\"600\" height=\"337\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>\u8ee2\u5199\u30cf\u30d6\u304c\u5b58\u5728\u3059\u308b\u72b6\u6cc1\u3067\u306e\u30af\u30ed\u30de\u30c1\u30f3\u306e\u52d5\u304d\u306e\u518d\u73fe\uff08\u540d\u53e4\u5c4b\u5927\u5b66\u5de5\u5b66\u7814\u7a76\u79d1\u30fb\u7b39\u4e95\u7406\u751f\u6559\u6388\u30b0\u30eb\u30fc\u30d7\u63d0\u4f9b\u3001Nagashima <em>et al.<\/em>, 2019\u3088\u308a\uff09<\/p>\n<p><iframe loading=\"lazy\" src=\"https:\/\/www.youtube.com\/embed\/Db19FENaaPw\" width=\"600\" height=\"337\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>\u8ee2\u5199\u30cf\u30d6\u304c\u5b58\u5728\u3057\u306a\u3044\u72b6\u6cc1\u3067\u306e\u30af\u30ed\u30de\u30c1\u30f3\u306e\u52d5\u304d\u306e\u518d\u73fe\uff08\u540d\u53e4\u5c4b\u5927\u5b66\u5de5\u5b66\u7814\u7a76\u79d1\u30fb\u7b39\u4e95\u7406\u751f\u6559\u6388\u30b0\u30eb\u30fc\u30d7\u63d0\u4f9b\u3001Nagashima <em>et al.<\/em>, 2019\u3088\u308a\uff09<\/p>\n<p>\u3053\u306e\u7d50\u679c\u304b\u3089\u3001\u8a08\u7b97\u6a5f\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u306b\u3088\u3063\u3066\u3082\u3001\u30af\u30ed\u30de\u30c1\u30f3\u306fRNAPII\u304c\u96c6\u307e\u3063\u305f\u5834\u6240\u3092\u30cf\u30d6\u3068\u3057\u3066\u3086\u308b\u3084\u304b\u306a\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u3092\u5f62\u6210\u3057\u3001\u675f\u7e1b\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u652f\u6301\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n<h3>\u8ee2\u5199\u3068\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u7406\u89e3\u306b\u5411\u3051\u3066<\/h3>\n<p>\u4eca\u56de\u79c1\u305f\u3061\u306f\u3001\u5358\u4e00\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3068\u8a08\u7b97\u6a5f\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u3092\u7528\u3044\u3066\u3001\u751f\u304d\u305f\u30d2\u30c8\u7d30\u80de\u6838\u306e\u30b2\u30ce\u30e0\u30ef\u30a4\u30c9\u306a\u30af\u30ed\u30de\u30c1\u30f3\u306e\u52d5\u304d\u3092\u8abf\u3079\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001RNAPII\u306b\u3088\u308b\u8ee2\u5199\u3092\u963b\u5bb3\u3059\u308b\u3068\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u304c\u4e0a\u6607\u3059\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u3053\u3068\u304b\u3089\u3001\u30af\u30ed\u30de\u30c1\u30f3\u306f\u3001RNAPII\u304c\u96c6\u307e\u308a\u8ee2\u5199\u304c\u8d77\u3053\u308b\u5834\u6240\u3092\u30cf\u30d6\u3068\u3057\u3066\u3086\u308b\u3084\u304b\u306a\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u3092\u5f62\u6210\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u8ee2\u5199\u306e\u30cf\u30d6\u306f\u3001RNAPII\u304a\u3088\u3073\u8ee2\u5199\u95a2\u9023\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5c40\u6240\u7684\u306b\u9ad8\u6fc3\u5ea6\u306b\u4fdd\u3061\u3001\u8ee2\u5199\u3059\u308b\u30af\u30ed\u30de\u30c1\u30f3\u9818\u57df\u306e\u52d5\u304d\u3092\u6291\u3048\u308b\u3053\u3068\u3067\u8ee2\u5199\u52b9\u7387\u306e\u5411\u4e0a\u306b\u5bc4\u4e0e\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/p>\n<p>\u4eca\u56de\u306e\u79c1\u305f\u3061\u306e\u77e5\u898b\u306f\u8ee2\u5199\u306e\u5206\u91ce\u3067\u53e4\u304f\u304b\u3089\u63d0\u5531\u3055\u308c\u3066\u304d\u305f\u3001\u30b2\u30ce\u30e0DNA\u304c\u96c6\u307e\u3063\u3066\u8ee2\u5199\u304c\u8d77\u3053\u308b\u3068\u3044\u3046\u300c\u8ee2\u5199\u30d5\u30a1\u30af\u30c8\u30ea\u30fc\u300d\u306e\u6982\u5ff5\u3068\u3088\u304f\u4e00\u81f4\u3057\u307e\u3059\u3002\u307e\u305f\u6700\u8fd1\u3001RNAPII\u3084\u8ee2\u5199\u56e0\u5b50\u304c\u6838\u5185\u3067\u5c40\u6240\u7684\u306b\u96c6\u307e\u3063\u3066\u6db2\u6ef4\u3092\u4f5c\u308a\u3001\u8ee2\u5199\u3092\u6d3b\u767a\u5316\u3059\u308b\u3068\u3044\u3046\u4eee\u8aac\u3082\u63d0\u5531\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u8ee2\u5199\u306e\u30cf\u30d6\u306f\u6db2\u6ef4\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u8ee2\u5199\u56e0\u5b50\u306e\u6db2\u6ef4\u3068\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u52d5\u304d\u306e\u95a2\u4fc2\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3053\u3068\u306f\u4eca\u5f8c\u306e\u8ab2\u984c\u3068\u3044\u3048\u308b\u3067\u3057\u3087\u3046\u3002<\/p>\n<p>\u7d30\u80de\u306e\u591a\u304f\u306e\u6d3b\u52d5\u306f\u3001\u30b2\u30ce\u30e0DNA\u4e0a\u306e\u907a\u4f1d\u60c5\u5831\u3092\u8aad\u307f\u51fa\u3059\u8ee2\u5199\u304b\u3089\u59cb\u307e\u308a\u307e\u3059\u3002\u8fd1\u5e74\u3001\u8ee2\u5199\u306e\u7570\u5e38\u306b\u3088\u3063\u3066\u3001\u30ac\u30f3\u5316\uff65\u7d30\u80de\u6b7b\u3001\u3055\u3089\u306b\u306f\u5206\u5316\u7570\u5e38\u306a\u3069\u3001\u3055\u307e\u3056\u307e\u306a\u7d30\u80de\u969c\u5bb3\u3092\u3082\u305f\u3089\u3059\u3053\u3068\u304c\u308f\u304b\u3063\u3066\u304d\u307e\u3057\u305f\u3002\u672c\u7814\u7a76\u306e\u6210\u679c\u306b\u3088\u3063\u3066\u3001\u3053\u306e\u3088\u3046\u306a\u7d30\u80de\u306e\u7570\u5e38\u3084\u95a2\u9023\u75be\u60a3\u306e\u7406\u89e3\u304c\u9032\u3080\u3053\u3068\u3082\u671f\u5f85\u3055\u308c\u307e\u3059\u3002<\/p>\n<p><b>\u53c2\u8003\u6587\u732e<\/b><br \/>\n\u30fb<a href=\"http:\/\/jcb.rupress.org\/content\/early\/2019\/02\/28\/jcb.201811090\">Nagashima, R., Hibino, K., Ashwin, S.S., Babokhov, M., Fujishiro, S., Imai, R., Nozaki, T., Tamura, S., Tani, T., Kimura, H., Shribak, M., Kanemaki, M.T., Sasai, M., Maeshima, K. &#8220;Single nucleosome imaging reveals loose genome chromatin networks via active RNA polymerase II&#8221; <em>Journal of Cell Biology<\/em>, 218, 1511-1530. (2019) <\/a><br \/>\n\u30fb<a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0955067418301881\">Maeshima, K., Ide, S., Babokhov, M. &#8220;Dynamic chromatin organization without the 30-nm fiber&#8221; <em>Current Opinion in Cell Biology<\/em>, 58, 95-104 (2019) <\/a><br \/>\n\u30fb<a href=\"http:\/\/www.cell.com\/molecular-cell\/fulltext\/S1097-2765(17)30445-8\">Nozaki, T., Imai, R., Tanbo, M., Nagashima, R., Tamura, S., Tani, T., Joti, Y., Tomita, M., Hibino, K., Wendt, K.S., Okada, Y., Nagai, T., Maeshima, K. &#8220;Dynamic organization of chromatin domains revealed by super-resolution live-cell imaging&#8221; <em>Molecular Cell<\/em>, 67, 282-293. (2017)<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30cc\u30af\u30ec\u30aa\u30bd\u30fc\u30e0\u306e\u3075\u308b\u307e\u3044\u3068\u306f\uff1f \u79c1\u305f\u3061\u306e\u4f53\u306f\u7d0440\u5146\u500b\u306e\u7d30\u80de\u304b\u3089\u69cb\u6210\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u308c\u305e\u308c\u306e\u7d30\u80de\u306b\u306f\u3001\u751f\u547d\u306e\u8a2d\u8a08\u56f3\u3068\u3082\u79f0\u3055\u308c\u308b\u5168\u9577\u7d042m\u306e\u30d2\u30c8\u30b2\u30ce\u30e0DNA\u304c\u53ce\u7d0d\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306eDNA\u306f\u76f4\u5f842nm\uff081 [&hellip;]<\/p>\n","protected":false},"author":396,"featured_media":10807,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[3],"tags":[98,82,15,85],"class_list":["post-10809","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-outcome","tag-98","tag-82","tag-biology","tag-85","has_thumb"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/posts\/10809","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/users\/396"}],"replies":[{"embeddable":true,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=10809"}],"version-history":[{"count":12,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/posts\/10809\/revisions"}],"predecessor-version":[{"id":10898,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/posts\/10809\/revisions\/10898"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=\/wp\/v2\/media\/10807"}],"wp:attachment":[{"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=10809"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=10809"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/academist-cf.com\/journal\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=10809"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}